Nojima Y, Enzan H, Hayashi Y, Nakayama H, Kiyoku H, Hiroi M, Mori K
Department of Neurosurgery, Kochi Medical School, Japan.
Pathol Int. 1998 Feb;48(2):115-25. doi: 10.1111/j.1440-1827.1998.tb03880.x.
To investigate the pathogenesis of congenital hydrocephalus the brains of HTX rats aged between 16 days and 4 weeks and the brains of normal Wistar rats of the same ages were examined. In the fetal HTX rat brains, the lateral ventricles were symmetrically dilated from 20 days of gestation. The neuroepithelium bordering the ventricles showed thinning with cellular disarrangement and deformity. Similar neuroepithelial abnormalities were also found in the lateral ventricles of the HTX rat brain with no macroscopic signs of hydrocephalus at 20 days of gestation. The neuroepithelium showed flattening of the cells, widening of the intercellular spaces, formation of microvilli on the detached lateral cell surfaces, and frequent macrophage infiltration. On the other hand, the neuroepithelial cells of the third ventricle and the aqueduct were affected less severely or showed no significant abnormalities. Immunohistochemically, most of the neuroepithelium and ependyma of the lateral ventricles were positive for vimentin in both prenatal and postnatal hydrocephalic HTX rats, while a small number or none of those in normal control rats were positive. These morphological changes suggested that preferential involvement of the lateral ventricular neuroepithelium might be closely associated with the pathogenesis of congenital hydrocephalus in HTX rats.
为研究先天性脑积水的发病机制，对16日龄至4周龄的HTX大鼠及同龄正常Wistar大鼠的脑进行了检查。在胎龄20天的HTX大鼠脑中，侧脑室自妊娠20天起呈对称性扩张。脑室周围的神经上皮变薄，细胞排列紊乱且畸形。在妊娠20天无脑积水宏观体征的HTX大鼠脑侧脑室中也发现了类似的神经上皮异常。神经上皮表现为细胞扁平、细胞间隙增宽、外侧细胞表面出现微绒毛以及巨噬细胞频繁浸润。另一方面，第三脑室和导水管的神经上皮细胞受影响较轻或无明显异常。免疫组织化学显示，产前和产后脑积水的HTX大鼠侧脑室的大多数神经上皮和室管膜波形蛋白呈阳性，而正常对照大鼠中只有少数或没有呈阳性。这些形态学变化表明，侧脑室神经上皮的优先受累可能与HTX大鼠先天性脑积水的发病机制密切相关。
