AP3衣被复合体在内体形成突触小泡过程中的作用。
A function for the AP3 coat complex in synaptic vesicle formation from endosomes.
作者信息
Faúndez V, Horng J T, Kelly R B
机构信息
Department of Biochemistry and Biophysics and Hormone Research Institute, University of California, San Francisco 94143-0534, USA.
出版信息
Cell. 1998 May 1;93(3):423-32. doi: 10.1016/s0092-8674(00)81170-8.
Synaptic vesicles can be coated in vitro in a reaction that is ARF-, ATP-, and temperature-dependent and requires synaptic vesicle membrane proteins. The coat is largely made up of the heterotetrameric complex, adaptor protein 3, recently implicated in Golgi-to-vacuole traffic in yeast. Depletion of AP3 from brain cytosol inhibits small vesicle formation from PC12 endosomes in vitro. Budding from washed membranes can be reconstituted with purified AP3 and recombinant ARF1. We conclude that AP3 coating is involved in at least one pathway of small vesicle formation from endosomes.
突触小泡在体外可通过一种依赖于ADP核糖基化因子（ARF）、三磷酸腺苷（ATP）和温度且需要突触小泡膜蛋白的反应进行包被。这种包被主要由异源四聚体复合物——衔接蛋白3组成，该蛋白最近被认为参与酵母中从高尔基体到液泡的运输过程。从脑细胞质中去除衔接蛋白3会抑制体外PC12内体形成小泡。用纯化的衔接蛋白3和重组ADP核糖基化因子1可重建从洗涤过的膜上出芽的过程。我们得出结论，衔接蛋白3包被至少参与内体形成小泡的一条途径。
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