DNA内部C.T错配的热力学
Thermodynamics of internal C.T mismatches in DNA.
作者信息
Allawi H T, SantaLucia J
机构信息
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
出版信息
Nucleic Acids Res. 1998 Jun 1;26(11):2694-701. doi: 10.1093/nar/26.11.2694.
Abstract
Thermodynamics of 23 oligonucleotides with internal single C.T mismatches were obtained by measuring UV absorbance as a function of temperature. Results from these 23 duplexes were combined with three measurements from the literature to derive nearest-neighbor thermodynamic parameters for seven linearly independent trimer sequences with internal C.T mismatches. The data show that the nearest-neighbor model is adequate for predicting thermodynamics of oligonucleotides with internal C.T with average deviations for Delta G degrees37, Delta H degrees, Delta S degrees and T m of 6.4%, 9.9%, 10.6%, and 1.9 degreesC respectively. C.T mismatches destabilize the duplex in all sequence contexts. The thermodynamic contribution of C. T mismatches to duplex stability varies weakly depending on the orientation of the mismatch and its context and ranges from +1.02 kcal/mol for GCG/CTC and CCG/GTC to +1.95 kcal/mol for TCC/ATG.
摘要
通过测量紫外吸光度随温度的变化，获得了23个具有内部单C.T错配的寡核苷酸的热力学数据。将这23个双链体的结果与文献中的三项测量结果相结合，推导出了七个具有内部C.T错配的线性独立三聚体序列的近邻热力学参数。数据表明，近邻模型足以预测具有内部C.T的寡核苷酸的热力学，对于ΔG°37、ΔH°、ΔS°和T m的平均偏差分别为6.4%、9.9%、10.6%和1.9℃。在所有序列背景下，C.T错配都会使双链体不稳定。C.T错配对双链体稳定性的热力学贡献随错配的方向及其背景而略有变化，范围从GCG/CTC和CCG/GTC的+1.02千卡/摩尔到TCC/ATG的+1.95千卡/摩尔。
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