SantaLucia J, Allawi H T, Seneviratne P A
Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA.
Biochemistry. 1996 Mar 19;35(11):3555-62. doi: 10.1021/bi951907q.
Thermodynamic data were determined from UV absorbance vs temperature profiles of 23 oligonucleotides. These data were combined with data from the literature for 21 sequences to derive improved parameters for the 10 Watson-Crick nearest neighbors. The observed trend in nearest-neighbor stabilities at 37 degrees C is GC > CG > GG > GA approximately GT approximately CA > CT > AA > AT > TA (where only the top strand is shown for each nearest neighbor). This trend suggests that both sequence and base composition are important determinants of DNA duplex stability. On average, the improved parameters predict deltaG degrees(37), deltaH degrees, deltaS degrees, and T(m) within 4%, 7%, 8%, and 2 degrees C, respectively. The parameters are optimized for the prediction of oligonucleotides dissolved in 1 M NaC1.
通过23种寡核苷酸的紫外吸光度与温度曲线测定了热力学数据。这些数据与来自文献的21个序列的数据相结合，以推导10种沃森-克里克最近邻碱基对的改进参数。在37摄氏度时观察到的最近邻碱基对稳定性趋势为GC > CG > GG > GA ≈ GT ≈ CA > CT > AA > AT > TA（其中每个最近邻碱基对仅显示顶部链）。这种趋势表明序列和碱基组成都是DNA双链稳定性的重要决定因素。平均而言，改进后的参数预测的ΔG°(37)、ΔH°、ΔS°和T(m)分别在4%、7%、8%和2摄氏度范围内。这些参数针对溶解在1 M NaCl中的寡核苷酸的预测进行了优化。
