Revello M G, Zavattoni M, Sarasini A, Percivalle E, Simoncini L, Gerna G
Institute of Infectious Diseases, University of Pavia, IRCCS Policlinico San Matteo, Italy.
J Infect Dis. 1998 May;177(5):1170-5. doi: 10.1086/515277.
Human cytomegalovirus (HCMV) was investigated in peripheral blood leukocytes (PBL) of 52 immunocompetent patients (40 pregnant women) with primary HCMV infection by quantitative determination of pp65 antigenemia, viremia, and leukoDNAemia. pp65 antigenemia was detected in 12 (57.1%) of 21, 4 (25%) of 16, and 0 of 10 patients examined 1, 2, and 3 months after onset, respectively. Viremia was detected in 5 (26.3%) of 19 patients during the first month only. LeukoDNAemia was detected in 20 of 20, 17 (89.5%) of 19, and 9 (47.3%) of 19 patients tested 1, 2, and 3 months after onset, respectively. Four (26.6%) of 15 patients were still DNAemia-positive at 4-6 months, whereas none were positive at >6 months. HCMV was not detected in PBL of 20 HCMV-immune donors or of 9 seropositive subjects with recurrent infection. Virus levels were low by all assays and did not correlate with clinical course of infection, intrauterine transmission, or severity of outcome. Invasive procedures in the presence of maternal leukoDNAemia did not seem to interfere with vertical transmission of HCMV infection.
通过定量测定pp65抗原血症、病毒血症和白细胞DNA血症，对52例具有原发性人巨细胞病毒（HCMV）感染的免疫功能正常患者（40例孕妇）的外周血白细胞（PBL）进行了研究。分别在发病后1、2和3个月检查的21例患者中有12例（57.1%）、16例患者中有4例（25%）以及10例患者中无1例检测到pp65抗原血症。仅在第一个月，19例患者中有5例（26.3%）检测到病毒血症。分别在发病后1、2和3个月检测的20例患者中有20例、19例患者中有17例（89.5%）以及19例患者中有9例（47.3%）检测到白细胞DNA血症。15例患者中有4例（26.6%）在4 - 6个月时仍为DNA血症阳性，而在>6个月时无1例阳性。在20例HCMV免疫供体或9例复发性感染的血清阳性受试者的PBL中未检测到HCMV。所有检测方法显示病毒水平均较低，且与感染的临床病程、宫内传播或结局严重程度无关。在产妇存在白细胞DNA血症的情况下进行侵入性操作似乎并未干扰HCMV感染的垂直传播。
