Bruyas J F, Martins-Ferreira C, Fiéni F, Tainturier D
Pathologie de la Reproduction, Ecole Nationale Vétérinaire de Nantes, France.
Equine Vet J Suppl. 1997 Dec(25):80-4. doi: 10.1111/j.2042-3306.1997.tb05107.x.
Seventeen horse embryos recovered on the sixth day after spontaneous ovulation were; 1) washed in PBS (n = 6), 2) treated with 1.5 M 1-2 propanediol (n = 6) or, 3) frozen and thawed using 1.5 M propanediol as the cryoprotectant (n = 5). After treatment, the embryos were incubated for 6 h in medium before they were fixed, serially sectioned and examined microscopically to count the total numbers of interphase, mitotic and pycnotic nuclei. Significant differences were measured only in the mean proportions of pycnotic cells (+/- s.d.), both between the control (9.2 +/- 7.3%) and frozen-thawed embryos (52.8 +/- 37.1%; P<0.05) and between the propanediol-treated (10.8 +/- 4.6%) and the frozen-thawed embryos (P<0.01). Propanediol appears to be minimally toxic to equine embryos but it is a poor cryoprotectant.
在自然排卵后第6天回收的17个马胚胎被分为：1）用磷酸盐缓冲液（PBS）冲洗（n = 6）；2）用1.5 M 1,2 - 丙二醇处理（n = 6）；3）以1.5 M丙二醇作为冷冻保护剂进行冷冻和解冻（n = 5）。处理后，胚胎在培养基中孵育6小时，然后固定、连续切片并进行显微镜检查，以计数间期、有丝分裂期和固缩核的总数。仅在固缩细胞的平均比例（±标准差）方面测量到显著差异，在对照组（9.2±7.3%）和冻融胚胎（52.8±37.1%；P<0.05）之间以及丙二醇处理组（10.8±4.6%）和冻融胚胎之间（P<0.01）。丙二醇对马胚胎的毒性似乎最小，但它是一种较差的冷冻保护剂。
