Lagneaux D, Huhtinen M, Koskinen E, Palmer E
Haras Nationaux-INRA Equine Reproduction, Nouzilly, France.
Equine Vet J Suppl. 1997 Dec(25):85-7. doi: 10.1111/j.2042-3306.1997.tb05108.x.
Equine embryos recovered on Day 6 after ovulation were cooled to +4 degrees C, or frozen with AFP alone or together with glycerol. Twenty embryos (140-200 microm in diameter) were randomly assigned to 6 treatment groups. In the first 3 groups, the embryos were cooled from room temperature to +4 degrees C at a rate of 3 degrees C/min and warmed again at a rate of 32 degrees C/min in a programmable freezer. In the second 3 groups, the embryos were frozen using a standard protocol, stored in liquid nitrogen for 5-7 days and then thawed in a 37 degrees C waterbath. After cooling/warming or freezing/thawing all the embryos were stained with DAPI. The percentage of dead cell area was significantly lower in the cooling groups than in the freezing groups and no significant differences were apparent between the cryoprotectants used in the study.
排卵后第6天回收的马胚胎被冷却至+4℃，或单独用抗冻蛋白（AFP）冷冻，或与甘油一起冷冻。将20个胚胎（直径140 - 200微米）随机分配到6个处理组。在前3组中，胚胎在程序降温仪中以3℃/分钟的速率从室温冷却至+4℃，并以32℃/分钟的速率再次升温。在后3组中，胚胎采用标准方案冷冻，在液氮中储存5 - 7天，然后在37℃水浴中解冻。在冷却/升温或冷冻/解冻后，所有胚胎都用4',6-二脒基-2-苯基吲哚（DAPI）染色。冷却组的死细胞面积百分比显著低于冷冻组，且研究中使用的冷冻保护剂之间没有明显差异。
