Aguilar J J, Woods G L, Miragaya M H, Olsen L M
Northwest Equine Reproduction Laboratory, Department of Animal and Veterinary Science, University of Idaho, Moscow 83844-2201, USA.
Equine Vet J Suppl. 1997 Dec(25):103-8. doi: 10.1111/j.2042-3306.1997.tb05112.x.
The object of this experiment was to estimate the number and type of living cells in oviductal masses of mares. Oviducts of abattoir mares were dissected, divided into 3 sections, and flushed individually. Oviductal masses were recovered from 220 of 250 mares and from 389 of 500 oviducts. A greater number of masses was recovered from the left than the right oviducts. A higher percentage of masses was recovered from the ampullary-isthmic junction than from the ampulla or isthmus. The number of masses increased slightly with increasing mare age and was weakly correlated with the number of unfertilised oocytes recovered per oviduct. Prepubertal mares had fewer recovered masses than anovulatory, early luteal phase, late luteal phase, or pregnant mares. Oviductal masses were classified morphologically as being branched, compact, or cumulus. Living cells were identified with a carboxy fluorescein diacetate stain and dead cells were identified with a propidium iodide fluorescent stain. In branched masses, the proportion of the surface area covered with total cells (live and dead) was 33.7 +/- 14.3%, and with only live cells was 6.2 +/- 7.3%. In compact masses, the proportion of the surface area covered with total cells was 42.4 +/- 21.2%, and with only live cells was 10.7 +/- 13.1%. The detection of living cells was confirmed by isolating and culturing cells. Cells cultured from cumulus masses were viable in 57.1% of wells, whereas cells from branched and compact masses were viable in only 18.1% and 17.7% of wells, respectively. In addition, more of the surface area of wells containing cells from cumulus masses were covered with cells, compared to wells containing cells from either branched or compact masses. Most cells appeared to be fibroblasts because 90-95% of cells from branched and compact masses were stained with a fibroblast cell marker.
本实验的目的是估计母马输卵管肿块中活细胞的数量和类型。解剖屠宰场母马的输卵管，将其分为3段，并分别冲洗。从250匹母马中的220匹以及500条输卵管中的389条中回收了输卵管肿块。从左输卵管回收的肿块数量多于右输卵管。从壶腹-峡部连接处回收的肿块百分比高于从壶腹或峡部回收的百分比。肿块数量随母马年龄的增加而略有增加，并且与每条输卵管中回收的未受精卵母细胞数量呈弱相关。青春期前的母马回收的肿块比无排卵、黄体早期、黄体晚期或怀孕母马少。输卵管肿块在形态上分为分支状、致密状或卵丘状。用羧基荧光素二乙酸酯染色鉴定活细胞，用碘化丙啶荧光染色鉴定死细胞。在分支状肿块中，总细胞（活细胞和死细胞）覆盖的表面积比例为33.7±14.3%，仅活细胞覆盖的表面积比例为6.2±7.3%。在致密状肿块中，总细胞覆盖的表面积比例为42.4±21.2%，仅活细胞覆盖的表面积比例为10.7±13.1%。通过分离和培养细胞证实了活细胞的检测。从卵丘状肿块培养的细胞在57.1%的孔中存活，而从分支状和致密状肿块培养的细胞分别仅在18.1%和17.7%的孔中存活。此外，与含有分支状或致密状肿块细胞的孔相比，含有卵丘状肿块细胞的孔中有更多的表面积被细胞覆盖。大多数细胞似乎是成纤维细胞，因为来自分支状和致密状肿块的细胞中有90 - 95%被成纤维细胞标记物染色。
