Lantz K C, Enders A C, Liu I K
Department of Cell Biology and Human Anatomy, University of California, Davis 95616, USA.
Anat Rec. 1998 Dec;252(4):568-79. doi: 10.1002/(SICI)1097-0185(199812)252:4<568::AID-AR7>3.0.CO;2-T.
In addition to the unique feature of retention of unfertilized ova, the oviducts of mares frequently contain large intraluminal masses with a fibrillar component and some cells. The aim of this study was to identify the cells and examine their relationship to the extracellular components of these masses. Intraluminal masses were examined both in situ and flushed from the oviducts. The nature of the contained cells and their relationship to the fibrils were examined by light microscopy and by transmission and scanning electron microscopy. In some mares the large masses distended the oviduct, but neither loss of the oviductal epithelium nor damage to this epithelium was seen. Electron microscopy verified that the principal cellular component was fibroblasts, and that the fibrils were type I collagen. Collagen masses collected shortly after ovulation frequently contained viable fibroblasts with collagen fibrils associated with their cell surfaces and with surface clefts. Although such collagen masses were present in pregnant and nonpregnant mares, masses with viable fibroblasts were chronologically associated with recent ovulation. It was concluded that connective tissue drawn into the oviduct at ovulation is retained, and collagen synthesis continues at least for a few days. Although the fibroblasts eventually disintegrate, the collagen remains and may in some cases aggregate within the oviductal lumen to the extent that oviductal transport and embryonic viability could be affected.
除了保留未受精卵这一独特特征外，母马的输卵管内常常含有带有纤维成分和一些细胞的大的管腔内团块。本研究的目的是鉴定这些细胞，并研究它们与这些团块细胞外成分的关系。对管腔内团块进行了原位检查，并从输卵管中冲洗出来进行研究。通过光学显微镜、透射电子显微镜和扫描电子显微镜检查所含细胞的性质及其与纤维的关系。在一些母马中，这些大的团块使输卵管扩张，但未观察到输卵管上皮的缺失或损伤。电子显微镜证实主要细胞成分是成纤维细胞，纤维是I型胶原。排卵后不久收集的胶原团块常常含有活的成纤维细胞，其细胞表面和表面裂隙处有胶原纤维。虽然在怀孕和未怀孕的母马中都存在这样的胶原团块，但含有活的成纤维细胞的团块在时间上与近期排卵相关。得出的结论是，排卵时进入输卵管的结缔组织被保留下来，胶原合成至少持续几天。虽然成纤维细胞最终会解体，但胶原会保留下来，在某些情况下可能会在输卵管腔内聚集，以至于影响输卵管运输和胚胎活力。
