Miragaya M H, Woods G L, Losinno L
Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Argentina.
Equine Vet J Suppl. 1997 Dec(25):109-12. doi: 10.1111/j.2042-3306.1997.tb05113.x.
The occurrence of uterine and oviductal inflammation, and fertilisation rates, were measured on Day 3 post ovulation in inseminated mares that had either exhibited intrauterine lumenal fluid during a previous dioestrus (Experiment 1) or had acute endometritis induced by intrauterine infusion of 1% glycogen (Experiment 2). Endometritis was assessed by uterine cytology and histology whereas oviductal inflammation was measured histologically. Fertilisation rates were calculated from the percentage of cleaved ova recovered by retrograde flushing of the oviducts. Mares with or without pre-existing uterine fluid during dioestrus that were inseminated showed a higher incidence of endometritis than control mares without pre-existing uterine fluid that were not inseminated (n = 7 mares/group). However, inseminated mares with uterine fluid did not show a higher incidence of endometritis than inseminated mares without uterine fluid. Mares with or without pre-existing uterine fluid showed a higher incidence of endometritis than salpingitis and these 2 groups of mares showed equivalent rates of fertilisation and oviductal oocyte recovery. Mares inseminated with semen alone or semen following 1% glycogen treatment had a higher incidence of endometritis than control noninseminated mares (n = 17 mares/group) but mares that received semen plus 1% glycogen did not show a higher incidence of endometritis than mares that received semen alone. Both these groups of mares showed a higher incidence of endometritis than salpingitis and those that received semen plus 1% glycogen showed an equal recovery rate of recently ovulated ova but a lower fertilisation rate than the mares that received semen alone.
在排卵后第3天，对授精母马的子宫和输卵管炎症以及受精率进行了测量。这些母马要么在之前的间情期出现过子宫腔内积液（实验1），要么通过子宫内注入1%糖原诱导了急性子宫内膜炎（实验2）。通过子宫细胞学和组织学评估子宫内膜炎，而输卵管炎症则通过组织学测量。受精率根据通过输卵管逆行冲洗回收的受精卵百分比计算得出。在间情期有或没有预先存在子宫积液的授精母马，其子宫内膜炎的发生率高于没有预先存在子宫积液且未授精的对照母马（每组7匹母马）。然而，有子宫积液的授精母马子宫内膜炎的发生率并不高于没有子宫积液的授精母马。有或没有预先存在子宫积液的母马子宫内膜炎的发生率高于输卵管炎，并且这两组母马的受精率和输卵管卵母细胞回收率相当。单独用精液授精或在1%糖原处理后用精液授精的母马，其子宫内膜炎的发生率高于未授精的对照母马（每组17匹母马），但接受精液加1%糖原的母马子宫内膜炎发生率并不高于单独接受精液的母马。这两组母马的子宫内膜炎发生率均高于输卵管炎，并且接受精液加1%糖原的母马最近排卵卵母细胞的回收率相同，但受精率低于单独接受精液的母马。
