Ding Y Q, Li J L, Lü B Z, Wang D, Zhang M L, Li J S
Department of Anatomy and K.K. Leung Brain Research Center, Fourth Military Medical University, Xi'an 710032, China.
Brain Res. 1998 May 4;792(1):149-53. doi: 10.1016/s0006-8993(98)00205-4.
Co-localization of mu-opioid receptor (MOR)-like immunoreactivity (-LI) with substance P (SP)-LI, calcitonin gene-related peptide (CGRP)-LI and nitric oxide synthase (NOS)-LI in the nodose, petrosal and jugular ganglia was examined in the rat by a double immunofluorescence histochemical method. About 0.6%, 41% and 95% of neurons with MOR-LI, respectively, in the nodose, petrosal and jugular ganglia showed SP-LI; about 2%, 51% and 66% of MOR-like immunoreactive neurons displayed CGRP-LI in the nodose, petrosal and jugular ganglia, respectively. In addition, about 59% of MOR-like immunoreactive neurons in the nodose ganglia displayed NOS-LI, whereas no NOS-LI was detected in the petrosal or jugular ganglion. These data provide evidence for co-localization of MOR-LI with SP-LI, CGRP-LI and NOS-LI in the vagal and glossopharyngeal afferent neurons, and suggest that MOR may regulate the release of SP, CGRP and nitric oxide from the visceral primary afferent terminals in the nucleus of the solitary tract of the rat.
采用双重免疫荧光组织化学方法，在大鼠中检测了延髓、岩神经节和颈静脉神经节中μ-阿片受体（MOR）样免疫反应性（-LI）与P物质（SP）-LI、降钙素基因相关肽（CGRP）-LI和一氧化氮合酶（NOS）-LI的共定位。在延髓、岩神经节和颈静脉神经节中，分别约有0.6%、41%和95%具有MOR-LI的神经元显示出SP-LI；在延髓、岩神经节和颈静脉神经节中，分别约有2%、51%和66%的MOR样免疫反应性神经元显示出CGRP-LI。此外，在延髓神经节中约59%的MOR样免疫反应性神经元显示出NOS-LI，而在岩神经节或颈静脉神经节中未检测到NOS-LI。这些数据为MOR-LI与SP-LI、CGRP-LI和NOS-LI在迷走神经和舌咽传入神经元中的共定位提供了证据，并表明MOR可能调节大鼠孤束核中内脏初级传入终末释放SP、CGRP和一氧化氮。
