Todd W M, Kafrawy A H, Newton C W, Brown C E
Indiana University School of Dentistry, Indianapolis 46202-5186, USA.
J Endod. 1997 Mar;23(3):152-7. doi: 10.1016/S0099-2399(97)80265-8.
This study investigated the presence of the putative peripheral neuromodulators Gamma-aminobutyric Acid (GABA) and Bombesin/Gastrin-Releasing Peptide (BN/GRP) in the human tooth pulp. Caries free and asymptomatic carious teeth were processed for paraffin embedding and sectioned at six microns. From each specimen, sections were stained with hematoxylin and eosin; other sections were subjected to Avidin-Biotin-Peroxidase Complex immunohistochemistry for GABA and BN/GRP. Sections of rat brain and small cell lung carcinoma served as positive controls. Results indicate the presence of specific GABA-like and BN/GRP-like immunoreactivity within the pulps of both normal and carious teeth. Overall staining for both ligands was significantly more intense within inflamed pulps. Based on their actions elsewhere, GABA and BN/GRP may play a role in the dental pulp as peripheral neuromodulators or as growth factors.
本研究调查了假定的外周神经调节因子γ-氨基丁酸（GABA）和蛙皮素/胃泌素释放肽（BN/GRP）在人牙髓中的存在情况。将无龋和无症状龋牙进行石蜡包埋，并切成6微米厚的切片。从每个标本中，切片用苏木精和伊红染色；其他切片进行抗生物素蛋白-生物素-过氧化物酶复合物免疫组织化学检测GABA和BN/GRP。大鼠脑和小细胞肺癌的切片作为阳性对照。结果表明，在正常和龋牙的牙髓中均存在特异性的GABA样和BN/GRP样免疫反应性。两种配体在炎症牙髓中的总体染色明显更强。基于它们在其他部位的作用，GABA和BN/GRP可能作为外周神经调节因子或生长因子在牙髓中发挥作用。
