Westergaard O, Marcker K A, Leer J C
Eur J Biochem. 1978 May;86(1):255-60. doi: 10.1111/j.1432-1033.1978.tb12306.x.
Replicative intermediates of discrete size (approximately 41 S) are observed in the eukaryotic organism Tetrahymena pyriformis, when the organism is grown under defined physiological conditions. The intermediates (believed to represent replicons) are synthesized and accumulated over longer periods of time (less than 90 min), if the cells are treated with low concentrations of cycloheximide. Under these conditions the rate of total DNA synthesis is only slightly inhibited (less than 15%), while maturation of intermediate DNA into high-molecular-weight DNA is completely blocked (greater than 98%). Cycloheximide appears to inhibit the maturation process more specifically than other protein synthesis inhibitors. Studies of the accumulated intermediates on alkaline buoyant density gradients demonstrate that initiation of new putative replicons occurs during treatment with cycloheximide.
当梨形四膜虫这种真核生物在特定生理条件下生长时，会观察到大小离散（约41S）的复制中间体。如果用低浓度的环己酰亚胺处理细胞，这些中间体（被认为代表复制子）会在较长时间（少于90分钟）内合成并积累。在这些条件下，总DNA合成速率仅受到轻微抑制（少于15%），而中间体DNA成熟为高分子量DNA的过程则完全受阻（大于98%）。环己酰亚胺似乎比其他蛋白质合成抑制剂更特异性地抑制成熟过程。在碱性浮力密度梯度上对积累的中间体进行研究表明，在用环己酰亚胺处理期间会发生新的假定复制子的起始。
