梨形四膜虫无细胞提取物中蛋白质合成的起始
Initiation of protein synthesis in cell-free extracts of Tetrahymena pyriformis.
作者信息
Smith K E, David E T
出版信息
Biochem J. 1981 May 15;196(2):641-4. doi: 10.1042/bj1960641.
Abstract
Saturating concentrations of the initiation-specific inhibitors poly(I) and T-2 toxin inhibit protein synthesis by over 35% and cause ribosome 'run-off' from the polyribosomes. The elongation-specific inhibitor cycloheximide totally prevents protein synthesis and 'freezes' the ribosomes in the pattern of unincubated controls. These results prove that our Tetrahymena extracts are capable of protein-synthesis initiation, a conclusion which is confirmed by a 30% inhibition of synthesis by the mRNA cap analogue, 7-methylguanosine 5'-monophosphate.
摘要
起始特异性抑制剂多聚肌苷酸（poly(I)）和T-2毒素的饱和浓度可使蛋白质合成抑制超过35%，并导致核糖体从多核糖体上“脱落”。延伸特异性抑制剂环己酰亚胺完全阻止蛋白质合成，并使核糖体“定格”在未孵育对照组的模式。这些结果证明，我们的四膜虫提取物能够进行蛋白质合成起始，这一结论通过mRNA帽类似物7-甲基鸟苷5'-单磷酸对合成的30%抑制得到证实。
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