Webber K M, Wallace C A, Smith A I, van den Buuse M
Baker Medical Research Institute, Prahran, Victoria, Australia.
J Cardiovasc Pharmacol. 1998;31 Suppl 1:S373-5. doi: 10.1097/00005344-199800001-00105.
We examined the interactions of endothelin (ET) with dopaminergic systems in rat brain. Using HPLC and radioimmunoassay, we found that striatum contained the highest levels of predominantly ET-1, whereas highest levels of predominantly ET-3 were found in the pituitary. Dopamine depletion in the striatum did not change the levels of immunoreactive ET, even though we have previously found a decrease in the density of ET receptors. In a comparison of spontaneously hypertensive rats (SHR) with Wistar-Kyoto (WKY) rats, ET levels were lower in cerebellum and medulla, with no difference in striatum or in other brain areas. In conclusion, ET is present in high levels in striatum, but these levels are not affected by dopamine depletion or in SHR.
我们研究了内皮素（ET）与大鼠脑中多巴胺能系统的相互作用。通过高效液相色谱法（HPLC）和放射免疫测定法，我们发现纹状体中主要以ET-1的含量最高，而垂体中主要以ET-3的含量最高。纹状体中的多巴胺耗竭并没有改变免疫反应性ET的水平，尽管我们之前发现ET受体密度有所降低。在自发性高血压大鼠（SHR）与Wistar-Kyoto（WKY）大鼠的比较中，小脑和延髓中的ET水平较低，纹状体或其他脑区则没有差异。总之，ET在纹状体中含量很高，但这些水平不受多巴胺耗竭或SHR的影响。
