Mercado R C, Moussy F
University of Connecticut Health Center, Center for Biomaterials, Farmington 06030-1615, USA.
Biosens Bioelectron. 1998 Feb 1;13(2):133-45. doi: 10.1016/s0956-5663(97)00112-7.
The effects of the biological environment on the perfluorosulfonated ionomer Nafion membrane were investigated. Nafion membranes thermally annealed at 120 degrees C and kept in culture medium or implanted subcutaneously in rats showed extensive cracking after 4 weeks. In membranes annealed at 150 degrees C, cracking was reduced, but not eliminated. Deposits of calcium phosphates in the membrane were identified. These deposits appeared to be responsible for the cracking of the membranes, but the precise mechanism was unclear. The permeability to glucose of Nafion membranes annealed at 120 degrees C increased at 1 week and then decreased during the 3 following weeks. However, the cracking, protein adhesion, and mineralization of the membranes made the results difficult to interpret. This study revealed that mineralization of Nafion occurs in the biological environment, resulting in cracking and changes in permeability. Modifications to prevent the mineralization of Nafion are necessary to make it suitable for use in the implantable glucose sensor.
研究了生物环境对全氟磺酸离子交换膜Nafion膜的影响。在120℃下进行热退火并保存在培养基中或皮下植入大鼠体内的Nafion膜在4周后出现大量裂纹。在150℃下退火的膜中，裂纹减少但未消除。在膜中鉴定出磷酸钙沉积物。这些沉积物似乎是导致膜破裂的原因，但确切机制尚不清楚。在120℃下退火的Nafion膜对葡萄糖的渗透性在第1周增加，随后在接下来的3周内下降。然而，膜的破裂、蛋白质粘附和矿化使得结果难以解释。这项研究表明，Nafion在生物环境中发生矿化，导致破裂和渗透性变化。为使Nafion适用于可植入葡萄糖传感器，有必要对其进行改性以防止矿化。
