Moussy F, Jakeway S, Harrison D J, Rajotte R V
Department of Chemistry, University of Alberta, Edmonton, Canada.
Anal Chem. 1994 Nov 15;66(22):3882-8. doi: 10.1021/ac00094a007.
In this paper we report that curing at 120 degrees C can be used to improve the in vivo durability of a miniaturized glucose sensor with an outer coating of the Dupont perfluorinated ionomer, Nafion. Sensors based on glucose oxidase trapped in an albumin/glutaraldehyde matrix were able to withstand curing at 120 degrees C without noticeable change in electrode sensitivity (+/- 22% SD). Curing above 120 degrees C caused a gradual decline in sensitivity, with no sensitivity seen at 170 degrees C. Curing Nafion at 120 degrees C eliminated ascorbic acid and urea interferences and improved selectivity for glucose against uric acid and acetaminophen, compared to room temperature-cured Nafion coatings. The Nafion film reduced O2 demand by the sensor, so the signal was O2 independent across a partial pressure range of 8-140 mmHg. Several of the fully assembled, heat-cured, needle-type glucose sensors remained functional for at least 10 days after subcutaneous implantation in dogs, without degradation of their sensitivity (average 3 nA/mM in vivo at 37 degrees C and 6 nA/mM in vitro at 37 degrees C).
在本文中，我们报告称，在120摄氏度下固化可用于提高带有杜邦全氟离子聚合物Nafion外涂层的微型葡萄糖传感器的体内耐用性。基于捕获在白蛋白/戊二醛基质中的葡萄糖氧化酶的传感器能够承受120摄氏度的固化，电极灵敏度无明显变化（标准偏差为±22%）。在120摄氏度以上固化会导致灵敏度逐渐下降，在170摄氏度时则无灵敏度。与室温固化的Nafion涂层相比，在120摄氏度下固化Nafion可消除抗坏血酸和尿素干扰，并提高对葡萄糖相对于尿酸和对乙酰氨基酚的选择性。Nafion膜降低了传感器对氧气的需求，因此在8 - 140 mmHg的分压范围内信号与氧气无关。几只完全组装好、经过热固化的针型葡萄糖传感器在皮下植入狗体内后至少能保持功能10天，其灵敏度无下降（在37摄氏度时体内平均为3 nA/mM，在37摄氏度时体外为6 nA/mM）。
