Beye M, Poch A, Burgtorf C, Moritz R F, Lehrach H
Institut für Okologie und Biologie, Technische Universität Berlin, Germany.
Genomics. 1998 Apr 15;49(2):317-20. doi: 10.1006/geno.1998.5253.
We present a gridded genomic library of the honey-bee (Apis mellifera) for comparative and basic genetic study of the honeybee genome. The library will be established as a "Reference Library" system, and clones as well as data will be shared with the entire scientific community. This will accelerate the molecular level of honeybee genetics, combining the efforts of different laboratories. Because of male haploidy and the high rate of recombination, the honeybee is becoming a model organism for genomic studies of naturally occurring traits and behavioral genetics. The library consists of about 110,000 clones spotted at high density onto four filter membranes, representing 22 genome equivalents. Preliminary analysis using single-copy sequences revealed a positive clone number of the same order. The techniques for library generation and preliminary analysis as well as library access are described.
我们展示了一个蜜蜂（Apis mellifera）的网格化基因组文库，用于蜜蜂基因组的比较和基础遗传研究。该文库将作为一个“参考文库”系统建立，克隆以及数据将与整个科学界共享。这将加快蜜蜂遗传学在分子层面的研究，整合不同实验室的力量。由于雄性单倍体和高重组率，蜜蜂正成为研究自然性状和行为遗传学基因组的模式生物。该文库由约11万个高密度点样于四张滤膜上的克隆组成，代表22个基因组当量。使用单拷贝序列的初步分析显示出相同数量级的阳性克隆。文中描述了文库构建、初步分析以及文库获取的技术。
