Kuwamura M, Yamate J, Kotani T, Takeuchi T, Sakuma S
Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Sakai, Japan.
Vet Pathol. 1998 May;35(3):223-6. doi: 10.1177/030098589803500309.
A 13-year-old male Shetland Sheepdog had a subcutaneous tumor in the left brachium. The tumor was removed and recurred several times at 5, 13, 16, 22, and 31 months after the initial presentation. Histologically, the removed nodules from the fourth resection were composed of neoplastic proliferation of round to fusiform cells, which possessed eosinophilic globules in their cytoplasm. The globules were periodic acid-Schiff positive and diastase resistant. Positive reactions for acid phosphatase were observed in the cytoplasm of the tumor cells. Ultrastructurally, these globules consisted of membrane-bound, dense structures containing dense granules, lucent vacuoles, and homogeneous materials. The recurrent tumors removed at the fifth resection consisted of spindle cell proliferation arranged in interlacing fascicles with wavy nuclei and containing a small number of cells with cytoplasmic globules. The tumor cells were immunoreactive to vimentin, S-100 protein, myelin basic protein, and neuron-specific enolase. The tumor was diagnosed as a peripheral nerve sheath tumor with eosinophilic cytoplasmic globules. These findings are unique for the histogenesis of granular cell tumors.
一只13岁的雄性设得兰牧羊犬左上肢有一个皮下肿瘤。该肿瘤被切除，在初次就诊后的5、13、16、22和31个月多次复发。组织学上，第四次切除的结节由圆形至梭形细胞的肿瘤性增殖组成，这些细胞的细胞质中含有嗜酸性小球。这些小球对过碘酸希夫反应呈阳性且耐淀粉酶。在肿瘤细胞的细胞质中观察到酸性磷酸酶呈阳性反应。超微结构上，这些小球由膜结合的致密结构组成，含有致密颗粒、透明空泡和均质物质。第五次切除的复发性肿瘤由梭形细胞增殖组成，这些细胞排列成交错的束状，核呈波浪状，并且含有少量带有细胞质小球的细胞。肿瘤细胞对波形蛋白、S-100蛋白、髓鞘碱性蛋白和神经元特异性烯醇化酶呈免疫反应。该肿瘤被诊断为伴有嗜酸性细胞质小球的周围神经鞘瘤。这些发现对于颗粒细胞瘤的组织发生来说是独特的。
