Semino C E, Allende M L, Bakkers J, Spaink H P, Robbins P P
Center of Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA.
Ann N Y Acad Sci. 1998 Apr 15;842:49-54. doi: 10.1111/j.1749-6632.1998.tb09631.x.
In this report we present data about the effect of the Rhizobium NodZ enzyme on zebrafish development. We injected zebrafish embryos with a plasmid expressing NodZ protein, and we confirmed that the enzyme is active and has chitin oligosaccharide fucosyltransferase (NodZ) activity in vitro. In addition, the embryos injected with the NodZ-expressing plasmid, but not with a control plasmid, showed malformations or bends in the tail, and in some cases shunted tail structures and fused somites. These results clearly indicate that the likely substrates for this enzyme, chitin oligosaccharides and free N-glycans, have essential functions during early vertebrate embryogenesis.
在本报告中，我们展示了有关根瘤菌NodZ酶对斑马鱼发育影响的数据。我们用表达NodZ蛋白的质粒注射斑马鱼胚胎，并证实该酶在体外具有活性且具有几丁质寡糖岩藻糖基转移酶（NodZ）活性。此外，注射了表达NodZ质粒而非对照质粒的胚胎出现了尾巴畸形或弯曲，在某些情况下还出现了尾巴结构分流和体节融合。这些结果清楚地表明，该酶可能的底物，即几丁质寡糖和游离N -聚糖，在早期脊椎动物胚胎发育过程中具有重要功能。
