Jakowec M W, Jackson-Lewis V, Chen X, Langston J W, Przedborski S
The Parkinson's Institute, Sunnyvale, Calif 94089-1605, USA.
Dev Neurosci. 1998;20(1):19-33. doi: 10.1159/000017295.
In situ hybridization and immunohistochemistry were used to characterize the expression pattern of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors during postnatal development in the rat basal ganglia. All subunit transcripts showed some degree of developmental regulation. GluR1 and GluR2 are expressed at high levels in the neonate with reduced expression in the adult. GluR3 and GluR4 are expressed at significantly lower levels in both neonates and adults and have much more modest degrees of reduced expression in adults as compared with GluR1 and GluR2. Analysis of the flip and flop transcript isoforms indicates that GluR1 flip and flop and GluR2 flip are the predominately expressed splice variants in adults. Observed changes in the expression of the AMPA receptor transcripts indicate that there are fundamental differences in the expression of these receptor subunits in adults and neonates. This phenomenon may play a significant role in the establishment of proper synaptic circuitry within the developing basal ganglia in early postnatal life as well as contributing to differences in susceptibility to injury and disease in the aging brain.
原位杂交和免疫组织化学被用于表征大鼠基底神经节出生后发育过程中α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体的表达模式。所有亚基转录本均显示出一定程度的发育调控。GluR1和GluR2在新生儿中高水平表达，在成体中表达降低。GluR3和GluR4在新生儿和成体中的表达水平均显著较低，与GluR1和GluR2相比，其在成体中的表达降低程度更为适度。对翻转和反翻转转录本异构体的分析表明，GluR1翻转和反翻转以及GluR2翻转是成体中主要表达的剪接变体。观察到的AMPA受体转录本表达变化表明，这些受体亚基在成体和新生儿中的表达存在根本差异。这种现象可能在出生后早期发育中的基底神经节内适当突触回路的建立中发挥重要作用，也有助于解释衰老大脑中对损伤和疾病易感性的差异。
