血浆和尿液中醛固酮及脱氧皮质酮的放射免疫测定法。
Radioimmunoassays for aldosterone and deoxycorticosterone in plasma and urine.
作者信息
Kurtz A B, Bartter F C
出版信息
Steroids. 1976 Jul;28(1):133-42. doi: 10.1016/0039-128x(76)90132-x.
Radioimmunoassays for aldosterone and deoxycorticosterone (DOC) are described in which a simple separation procedure using ammonium sulfate stabilization of bound steroid and extraction of free steroid into toluene scintillant allows an "in vial" assay without mechanical separation of the two phase system. Extraction and thin layer chromatographic methods for purification of aldosterone and DOC are free of solvent and plate blank effects. Normal values are given for unconjugated aldosterone and DOC in urine, for aldosterone and DOC in plasma and for aldosterone 18-glucuronide in urine.
本文描述了醛固酮和脱氧皮质酮（DOC）的放射免疫测定法，其中一种简单的分离程序利用硫酸铵稳定结合的类固醇并将游离类固醇萃取到甲苯闪烁剂中，从而实现“瓶内”测定，无需对两相系统进行机械分离。用于醛固酮和DOC纯化的萃取和薄层色谱方法不存在溶剂和板空白效应。文中给出了尿中未结合醛固酮和DOC、血浆中醛固酮和DOC以及尿中醛固酮18-葡萄糖醛酸苷的正常值。
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