Davis B M, Goodness T P, Soria A, Albers K M
Department of Anatomy, University of Kentucky College of Medicine, Lexington 40536-0084, USA.
Neuroreport. 1998 Apr 20;9(6):1103-7. doi: 10.1097/00001756-199804200-00027.
Previous studies have shown that transgenic mice over-expressing NGF in the skin have novel sympathetic 'basket-like' projections to sensory neurons similar to that seen in models of chronic pain. Since only a subset of sensory neurons in the NGF-transgenic mice received the sympathetic projections, we hypothesized that sympathetic sprouting was targeted to those neurons affected by increased levels of NGF. To test this, double-label immunohistochemistry for the NGF receptor (trkA) and sympathetic baskets was performed. Thirty-nine percent of all neurons in transgenic trigeminal ganglia were trkA-positive. Moreover, of the population of sensory neurons that received sympathetic input, 84% were trkA-positive. These results indicate that retrogradely transported NGF can induce and direct growth of sympathetic axons in vivo.
先前的研究表明，在皮肤中过度表达神经生长因子（NGF）的转基因小鼠对感觉神经元有类似慢性疼痛模型中所见的新型交感神经“篮状”投射。由于在NGF转基因小鼠中只有一部分感觉神经元接受交感神经投射，我们推测交感神经发芽是针对那些受NGF水平升高影响的神经元。为了验证这一点，我们进行了NGF受体（trkA）和交感神经篮的双标记免疫组织化学实验。转基因三叉神经节中所有神经元的39%为trkA阳性。此外，在接受交感神经输入的感觉神经元群体中，84%为trkA阳性。这些结果表明，逆行运输的NGF可以在体内诱导并引导交感神经轴突的生长。
