Marmigere F, Rage F, Tapia-Arancibia L, Arancibia S
Laboratory of Brain Plasticity, EP 628 CNRS, Université de Montpellier 2, France.
Neuroreport. 1998 Apr 20;9(6):1159-63. doi: 10.1097/00001756-199804200-00037.
We used a digoxigenin-UTP-labeled cRNA probe with in situ hybridization and Northern blot analysis to investigate the localization of brain derived neurotrophic factor (BDNF) mRNA and expression of its different transcripts in adult rat hypothalamus. As the BDNF gene is under the control of alternative multiple promoters, which provide tissue-specific gene expression, we studied whether these transcripts were expressed in adult hypothalamus. Our data revealed two novel sources of hypothalamic BDNF mRNA: the supraoptic and periventricular nuclei. In addition, we observed the expression of transcripts from exons, I, II and III as well as the presence of 1.6 and 4.2 kb BDNF mRNAs. Finally, our findings confirmed expression of mRNA encoding neurotrophins receptors in the hypothalamus.
我们使用地高辛配基-UTP标记的cRNA探针，通过原位杂交和Northern印迹分析，研究成年大鼠下丘脑源性神经营养因子（BDNF）mRNA的定位及其不同转录本的表达。由于BDNF基因受多个可变启动子的控制，这些启动子可提供组织特异性基因表达，因此我们研究了这些转录本在成年下丘脑中是否表达。我们的数据揭示了下丘脑BDNF mRNA的两个新来源：视上核和室周核。此外，我们观察到外显子I、II和III转录本的表达以及1.6和4.2 kb BDNF mRNA的存在。最后，我们的研究结果证实了下丘脑中编码神经营养因子受体的mRNA的表达。
