pZ402，一种改进的基于SV40的穿梭载体，含有一个无法与野生型p53相互作用的T抗原突变体。

pZ402, an improved SV40-based shuttle vector containing a T-antigen mutant unable to interact with wild-type p53.

作者信息

Kraus E, Strong L C, Tainsky M A

机构信息

Department of Tumor Biology, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.

出版信息

Gene. 1998 May 12;211(2):229-34. doi: 10.1016/s0378-1119(98)00121-8.

DOI:10.1016/s0378-1119(98)00121-8

PMID:9602136

Abstract

Shuttle vectors are useful tools for studying DNA replication and mutagenesis. SV40-based shuttle vectors are popular because of their ease of use and quick results. However, one complication with the use of SV40-based shuttle vectors is the interaction of cellular p53 protein with the T-antigen of SV40. Wild-type, but not mutant p53 has been shown to be involved in DNA replication and DNA repair. To address this concern, we have modified an SV40-based shuttle vector, pZ189, by exchanging the wt T-antigen for a mutant SV40 T-antigen, which is unable to bind with p53. This shuttle vector, pZ402, provides us with a tool to study DNA replication and genomic instability in cells with varying genetic backgrounds without interference from the interaction of T-antigen with p53.

摘要

穿梭载体是研究DNA复制和诱变的有用工具。基于SV40的穿梭载体因其使用方便和结果快速而广受欢迎。然而，使用基于SV40的穿梭载体的一个复杂问题是细胞p53蛋白与SV40的T抗原之间的相互作用。野生型而非突变型p53已被证明参与DNA复制和DNA修复。为了解决这一问题，我们对基于SV40的穿梭载体pZ189进行了改造，将野生型T抗原换成了一种无法与p53结合的突变型SV40 T抗原。这种穿梭载体pZ402为我们提供了一种工具，用于研究不同遗传背景细胞中的DNA复制和基因组不稳定性，而不受T抗原与p53相互作用的干扰。