Miranda S, Malán Borel I, Margni R
Departamento de Microbiologia, Inmunología y Biotecnología, Facultad de Farmacia Bioquímica, Universidad de Buenos Aires, Argentina.
Am J Reprod Immunol. 1998 May;39(5):341-9. doi: 10.1111/j.1600-0897.1998.tb00528.x.
The in vitro immunomodulating effect of placental culture supernatants (PSs) obtained from two H-2k x H-2d allogeneic crossbreedings, the CBA/J x DBA/2 abortion-prone mating combination, and the reproductively normal pregnancy CBA/J x BALB/c crossbreeding were compared, and the influence of previous deliveries was evaluated. The behavior of placentae obtained from CBA/J females with two previous pregnancies by BALB/c males was also investigated.
Supernatants of cultures of murine placentae were added to a mouse immunoglobulin (Ig) G1 hybridoma culture which produced anti-dinitrophenol (anti-DNP) antibodies. The quantity of monoclonal antibody produced, the nature of these antibodies, and the proliferation of the hybridoma cells were studied.
CBA/J x DBA/2 placental factors obtained from multiparous females induced a diminished asymmetric IgG antibody production without varying the quantity of antibody produced. In contrast, PSs obtained from the nonresorption-prone CBA/J x BALB/c mating combination with the same number of previous deliveries enhanced the production of both symmetric and asymmetric anti-DNP molecules and also increased the proportion of asymmetric blocking monoclonal antibodies (mAbs) synthesized by the hybridoma. Both of the PSs analyzed had induced similar inhibition of 3H-thymidine uptake. PSs obtained from the abortion-prone mating combination whose CBA/J females had two previous pregnancies by BALB/c males showed similar immunomodulating effects to those observed using multiparous CBA/J x BALB/c placentae.
We propose that the placenta produces soluble factors that participate in the regulation of antibody synthesis by the mother during gestation. Such a placental immunomodulating effect appears to be altered in the CBA/J x DBA/2 abortion-prone mating combination and could be corrected by previous pregnancies by BALB/c males. These observations suggest that placental factors would be relevant to the protection of the fetus and might play an important role in the immune equilibrium between mother and fetus. Asymmetric antibody production as a Th2 responsiveness was also discussed.
比较了从两种H-2k×H-2d同种异体杂交、CBA/J×DBA/2易流产交配组合以及生殖正常的妊娠CBA/J×BALB/c杂交中获得的胎盘培养上清液（PSs）的体外免疫调节作用，并评估了既往分娩的影响。还研究了由BALB/c雄性小鼠使CBA/J雌性小鼠经历两次妊娠后所获得胎盘的行为。
将小鼠胎盘培养物的上清液添加到产生抗二硝基苯酚（抗DNP）抗体的小鼠免疫球蛋白（Ig）G1杂交瘤培养物中。研究了产生的单克隆抗体的量、这些抗体的性质以及杂交瘤细胞的增殖情况。
从经产雌性小鼠获得的CBA/J×DBA/2胎盘因子导致不对称IgG抗体产生减少，但不改变产生的抗体量。相比之下，从既往分娩次数相同的不易吸收的CBA/J×BALB/c交配组合中获得的PSs增强了对称和不对称抗DNP分子的产生，并且还增加了杂交瘤合成的不对称阻断单克隆抗体（mAb）的比例。所分析的两种PSs均诱导了对3H-胸腺嘧啶核苷摄取的类似抑制作用。从CBA/J雌性小鼠经BALB/c雄性小鼠使之前经历两次妊娠的易流产交配组合中获得的PSs显示出与使用经产CBA/J×BALB/c胎盘所观察到的类似免疫调节作用。
我们提出胎盘产生可溶性因子，这些因子在妊娠期间参与调节母体的抗体合成。这种胎盘免疫调节作用在CBA/J×DBA/2易流产交配组合中似乎发生了改变，并且可以通过BALB/c雄性小鼠的既往妊娠来纠正。这些观察结果表明胎盘因子与胎儿的保护相关，并且可能在母胎免疫平衡中起重要作用。还讨论了作为Th2反应性的不对称抗体产生。
