Clerc S, Simonet P
Laboratoire d'Ecologie Microbienne du sol, UMR CNRS 5557, Université Lyon I, Villeurbanne Cedex, France.
Antonie Van Leeuwenhoek. 1998 Jan;73(1):15-23. doi: 10.1023/a:1000747310461.
The deliberate or accidental release of genetically engineered microorganisms (GEMs) in the environment has led to some questions concerning microbial survival, transfer of DNA to the indigenous microflora and environmental consequences. Amongst horizontal gene transfer mechanisms, conjugation is probably the most frequent in the environment. With the aim of evaluating risks associated with environmental release of GEMs and their engineered DNA, studies of conjugative gene transfer between a donor strain and indigenous microflora have been conducted. Such studies required the development of a donor counterselection system to prevent growth of donor cells on transconjugant selective plates. This review summarizes the known and potential donor counterselection systems.
基因工程微生物（GEMs）有意或意外释放到环境中引发了一些关于微生物存活、DNA向本地微生物群落转移以及环境后果的问题。在水平基因转移机制中，接合作用可能是环境中最常见的。为了评估与GEMs及其工程DNA环境释放相关的风险，已经开展了供体菌株与本地微生物群落之间接合基因转移的研究。此类研究需要开发一种供体反选择系统，以防止供体细胞在转接合子选择平板上生长。本综述总结了已知的和潜在的供体反选择系统。
