Fan P C
Institute of Parasitology, National Yangming University, Taipei, Taiwan, Republic of China.
Int J Parasitol. 1998 Apr;28(4):603-5. doi: 10.1016/s0020-7519(97)00215-4.
Metacercariae of Clonorchis sinensis were collected from freshwater fish (Pseudorasbora parva) after storage in a freezer at -12 degrees C for 10-20 days, in a freezer at -20 degrees for 3-7 days, or treating with heavy salt (fish/salt = 10 gm/3 gm) at 26 degrees for 5-15 days. The metacercariae from the frozen fish were inoculated into 30 rats and eight rabbits, and those from the salted fish into 33 rats. Metacercariae from freshwater fish stored at -12 degrees for 10-18 days or -20 degrees for 3-7 remained viable and infective. Moreover, those kept at the heavy salt concentration for 5-7 days remained viable and infective. These findings indicate that freezing or storing infected freshwater fish in heavy salt may not be effective in the prevention of clonorchiasis. However, it appears that refrigeration, or keeping the fish in salt for longer periods, may be suitable for prevention of the infection.
华支睾吸虫囊蚴取自淡水鱼（麦穗鱼），这些鱼分别在-12℃的冷冻箱中储存10 - 20天，在-20℃的冷冻箱中储存3 - 7天，或在26℃下用重盐处理（鱼/盐 = 10克/3克）5 - 15天。将冷冻鱼中的囊蚴接种到30只大鼠和8只兔子体内，将咸鱼中的囊蚴接种到33只大鼠体内。在-12℃储存10 - 18天或-20℃储存3 - 7天的淡水鱼中的囊蚴仍具有活力和感染性。此外，在重盐浓度下保存5 - 7天的囊蚴仍具有活力和感染性。这些发现表明，冷冻或用重盐储存受感染的淡水鱼可能无法有效预防华支睾吸虫病。然而，冷藏或将鱼在盐中保存更长时间似乎可能适合预防感染。
