[Separation of thiamine phosphoric esters on cation-exchange Saphadex].

A new method is suggested for separation of thiamine phosphoric esters (TPE) from biological materials. The aliquote of evaporated extract from tissue was applied to the column with Sephadex SE-C-25 (25X2.5), TTP passed through Sephadex with 0,005 M acetate buffer, pH 3.8-4.0, TDP was eluated with 0,05 M acetate buffer; TMP and T--1 M NaC1 in 1.0 M acetate buffer. Yield of each phosphoric ester was about 100%. The method allows separating thiamine phosphoric esters without preliminary purification of charcoal. It may be preferable to determine the content of TTP in tissue.