Parkhomenko Iu M, Rybina A A, Pol'shchak R B
Ukr Biokhim Zh. 1976 Apr-Jun;48(3):384-7.
A new method is suggested for separation of thiamine phosphoric esters (TPE) from biological materials. The aliquote of evaporated extract from tissue was applied to the column with Sephadex SE-C-25 (25X2.5), TTP passed through Sephadex with 0,005 M acetate buffer, pH 3.8-4.0, TDP was eluated with 0,05 M acetate buffer; TMP and T--1 M NaC1 in 1.0 M acetate buffer. Yield of each phosphoric ester was about 100%. The method allows separating thiamine phosphoric esters without preliminary purification of charcoal. It may be preferable to determine the content of TTP in tissue.
提出了一种从生物材料中分离硫胺磷酸酯（TPE）的新方法。将组织蒸发提取物的等分试样应用于装有Sephadex SE-C-25（25X2.5）的柱上，硫胺三磷酸（TTP）用0.005M乙酸盐缓冲液（pH 3.8 - 4.0）通过Sephadex，硫胺二磷酸（TDP）用0.05M乙酸盐缓冲液洗脱；硫胺单磷酸（TMP）和硫胺用1.0M乙酸盐缓冲液中的1M氯化钠洗脱。每种磷酸酯的产率约为100%。该方法无需对活性炭进行预纯化即可分离硫胺磷酸酯。对于测定组织中TTP的含量可能更可取。
