Tunçtan B, Okur H, Calişir C H, Abacioğlu H, Cakici I, Kanzik I, Abacioğlu N
Department of Pharmacology, Faculty of Pharmacy, Gazi University, Ankara, Turkey.
Pharmacol Res. 1998 Mar;37(3):219-26. doi: 10.1006/phrs.1997.0284.
The aim of the present study was to determine the NO production by human cultured macrophages (m phi) and to compare the NO production between healthy subjects and patients with active pulmonary tuberculosis. The bioassay method was used for assessment of validation. Lipopolysaccharide (125 ng ml-1)-activated m phi from healthy and diseased subjects released a substantial amount of NO. NO synthase inhibitor, NG-nitro-L-arginine methyl ester, (0.1 mmol l-1) suppressed NO synthesis significantly in m phi of healthy subjects. Nitrite formation measured by the diazotization method in the supernatants taken from cultured m phi of tuberculous patients were significantly lower than the healthy subjects. The supernatants obtained in both subjects caused relaxation of guinea-pig aorta reversed by methylene blue (10 mumol l-1). There was a significant difference between relaxations of healthy and diseased supernatants. Nitrite formation measured by the bioassay method in the supernatants taken from cultured m phi of tuberculous patients was significantly higher than the healthy subjects. It was concluded that NO production appeared to be decreased in tuberculosis. The reason for decreased production of NO in tuberculosis may be related to the interaction of several cytokines and/or eicosanoids by means of the disease related induction of immune reactions.
本研究的目的是测定人培养巨噬细胞（m phi）产生一氧化氮（NO）的情况，并比较健康受试者和活动性肺结核患者之间NO的产生情况。采用生物测定法进行有效性评估。来自健康和患病受试者的脂多糖（125 ng/ml）激活的m phi释放出大量NO。NO合酶抑制剂NG-硝基-L-精氨酸甲酯（0.1 mmol/l）显著抑制健康受试者m phi中的NO合成。通过重氮化法测定的结核患者培养m phi上清液中亚硝酸盐的形成明显低于健康受试者。两个受试者的上清液均引起豚鼠主动脉舒张，且可被亚甲蓝（10 μmol/l）逆转。健康和患病上清液的舒张作用存在显著差异。通过生物测定法测定的结核患者培养m phi上清液中亚硝酸盐的形成明显高于健康受试者。得出的结论是，结核病中NO的产生似乎减少。结核病中NO产生减少的原因可能与几种细胞因子和/或类花生酸通过疾病相关的免疫反应诱导的相互作用有关。
