Dumarey C H, Labrousse V, Rastogi N, Vargaftig B B, Bachelet M
Unité de Pharmacologie Cellulaire, Unité Associée Institut Pasteur-INSERM 285, Paris, France.
J Leukoc Biol. 1994 Jul;56(1):36-40. doi: 10.1002/jlb.56.1.36.
Infection with a virulent strain of Mycobacterium avium, but not with virulent Mycobacterium tuberculosis or avirulent Mycobacterium smegmatis, induced the formation of nitric oxide by human monocyte-derived macrophages. This process was not affected by lipopolysaccharide or cytokines such as interferon-gamma or tumor necrosis factor alpha. M. avium-induced nitric oxide production was significantly decreased by NG-monomethyl-L-arginine, a potent inhibitor of nitric oxide synthase activity, without any significant enhancement of intramacrophagic mycobacterial growth. Infection with all the three mycobacterial species induced a significant activation of phospholipase A2 activity of macrophages as evidenced by the increased release of thromboxane A2. Finally, nitric oxide production by human monocyte-derived macrophages required infection with live M. avium, as neither gamma-irradiated M. avium nor the subcellular fractions of this microorganism (cell wall, cytosol) were able to trigger nitric oxide synthesis.
感染强毒株鸟分枝杆菌可诱导人单核细胞衍生的巨噬细胞产生一氧化氮，而感染强毒力结核分枝杆菌或无毒耻垢分枝杆菌则不会。此过程不受脂多糖或细胞因子（如γ干扰素或肿瘤坏死因子α）影响。一氧化氮合酶活性的强效抑制剂NG-单甲基-L-精氨酸可显著降低鸟分枝杆菌诱导的一氧化氮生成，且巨噬细胞内分枝杆菌生长未显著增强。感染所有三种分枝杆菌均会显著激活巨噬细胞的磷脂酶A2活性，血栓素A2释放增加即证明了这一点。最后，人单核细胞衍生的巨噬细胞产生一氧化氮需要感染活的鸟分枝杆菌，因为经γ射线照射的鸟分枝杆菌及其亚细胞组分（细胞壁、胞质溶胶）均无法触发一氧化氮合成。
