从气调包装的鳕鱼片中分离出的腐败希瓦氏菌对氧化三甲胺还原作用的体外研究

In vitro study of TMAO reduction by Shewanella putrefaciens isolated from cod fillets packed in modified atmosphere.

作者信息

Boskou G, Debevere J

机构信息

Department of Food Technology and Nutrition, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, Belgium.

出版信息

Food Addit Contam. 1998 Feb-Mar;15(2):229-36. doi: 10.1080/02652039809374634.

DOI:10.1080/02652039809374634

PMID:9602930

Abstract

A strain of Shewanella putrefaciens was isolated from cod fillets in a modified atmosphere (60% CO2, 30% O2, 10% N2). The effect of pH on the growth of Shewanella putrefaciens and TMAO reduction was studied in fish extract with citrate buffer systems at pH values from 5.8 to 6.8. The effect of the modified atmosphere (CO2, O2) on the growth of S. putrefaciens and on the reduction of TMAO to TMA was studied using solid media from fish extract packed under variable mixtures of CO2, O2 and N2. All the samples were incubated at 7 degrees C for 7 days. Slow development of S. putrefaciens and no production of TMA was observed in fish extract buffered at pH 5.8. Inclusion of O2 in packaging atmosphere of solid fish extract, even at small proportions (10%), was inhibitory for the reduction of TMAO, while there was no effect of O2 on the growth of S. putrefaciens. Increased levels of CO2 (> or = 50%) in the atmosphere were inhibiting the growth of S. putrefaciens and consequently the production of TMA.

摘要

从处于气调包装（60%二氧化碳、30%氧气、10%氮气）的鳕鱼片中分离出一株腐败希瓦氏菌。在pH值为5.8至6.8的含柠檬酸盐缓冲系统的鱼提取物中，研究了pH对腐败希瓦氏菌生长及三甲胺氧化物（TMAO）还原的影响。使用在不同二氧化碳、氧气和氮气混合气体包装下的鱼提取物固体培养基，研究了气调包装（二氧化碳、氧气）对腐败希瓦氏菌生长以及TMAO还原为三甲胺（TMA）的影响。所有样品在7℃下孵育7天。在pH 5.8缓冲的鱼提取物中观察到腐败希瓦氏菌生长缓慢且未产生TMA。在固体鱼提取物的包装气氛中加入氧气，即使比例很小（10%），也会抑制TMAO的还原，而氧气对腐败希瓦氏菌的生长没有影响。气氛中二氧化碳水平升高（≥50%）会抑制腐败希瓦氏菌的生长，从而抑制TMA的产生。