Jørgensen B R, Huss H H
Technological Laboratory, Ministry of Fisheries, Technical University, Lyngby, Denmark.
Int J Food Microbiol. 1989 Aug;9(1):51-62. doi: 10.1016/0168-1605(89)90037-8.
A total of 101 cultures of H2S-producing organisms isolated from spoiling cod was studied. All cultures were identified as Shewanella putrefaciens. Two groups were distinguished on the basis of pattern of trimethylamine oxide reduction determined by conductance measurement, generation time at 25 degrees C and salt tolerance. The S. putrefaciens cultures were further characterized in a number of model experiments in order to examine the spoilage activity under various conditions. A good correlation between bacterial counts, detection time in conductance measurements and production of trimethylamine and off-odour was found. There were no differences in spoilage potential between strains of S. putrefaciens, and neither initial level of inoculum nor batch of cod used as substrate influenced the levels of bacterial count corresponding to a certain production of trimethylamine and off-odour.
对从变质鳕鱼中分离出的101株产硫化氢微生物培养物进行了研究。所有培养物均鉴定为腐败希瓦氏菌。根据通过电导率测量确定的氧化三甲胺还原模式、25℃下的代时和耐盐性区分出两组。为了研究在各种条件下的腐败活性，对腐败希瓦氏菌培养物在一些模型实验中进行了进一步表征。发现细菌计数、电导率测量中的检测时间与三甲胺和异味产生之间存在良好的相关性。腐败希瓦氏菌菌株之间的腐败潜力没有差异，用作底物的接种物初始水平和鳕鱼批次均不影响与一定量三甲胺和异味产生相对应的细菌计数水平。
