FtsI和FtsW定位于大肠杆菌的隔膜处。
FtsI and FtsW are localized to the septum in Escherichia coli.
作者信息
Wang L, Khattar M K, Donachie W D, Lutkenhaus J
机构信息
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66216, USA.
出版信息
J Bacteriol. 1998 Jun;180(11):2810-6. doi: 10.1128/JB.180.11.2810-2816.1998.
Abstract
The localization of FtsI (PBP3), a penicillin-binding protein specifically required for cell division in Escherichia coli, was investigated by immunofluorescence microscopy and found to localize to the septum. The localization of FtsI was not observed in ftsZ or ftsA mutants, indicating that it was dependent on the prior localization of these proteins. Addition of furazlocillin, a specific inhibitor of FtsI, prevented localization of FtsI even though FtsZ and FtsA localization occurred. Interestingly, the localization of FtsN was also prevented by furazlocillin. FtsZ displayed limited localization in furazlocillin-treated cells, whereas it was efficiently localized in FtsI-depleted cells. FtsW, another essential cell division protein, was also localized to the septum.
摘要
对大肠杆菌细胞分裂所特需的青霉素结合蛋白FtsI（PBP3）进行了免疫荧光显微镜定位研究，发现其定位于隔膜。在ftsZ或ftsA突变体中未观察到FtsI的定位，这表明它依赖于这些蛋白的先前定位。添加FtsI的特异性抑制剂呋唑西林，即使FtsZ和FtsA发生了定位，也会阻止FtsI的定位。有趣的是，呋唑西林也会阻止FtsN的定位。在经呋唑西林处理的细胞中，FtsZ显示出有限的定位，而在FtsI缺失的细胞中它能有效定位。另一种必需的细胞分裂蛋白FtsW也定位于隔膜。
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本文引用的文献
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Crystal structure of the bacterial cell-division protein FtsZ.细菌细胞分裂蛋白FtsZ的晶体结构。
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FtsN, a late recruit to the septum in Escherichia coli.FtsN，一种在大肠杆菌中较晚定位于隔膜的蛋白。
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