由GTP水解调节的FtsZ动态组装。
Dynamic assembly of FtsZ regulated by GTP hydrolysis.
作者信息
Mukherjee A, Lutkenhaus J
机构信息
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160, USA.
出版信息
EMBO J. 1998 Jan 15;17(2):462-9. doi: 10.1093/emboj/17.2.462.
Abstract
FtsZ forms a cytokinetic ring, designated the Z ring, that directs cytokinesis in prokaryotes. It has limited sequence similarity to eukaryotic tubulins and, like tubulin, it has GTPase activity and the ability to assemble into various structures including protofilaments, bundles and minirings. By using both electron microscopy and sedimentation, we demonstrate that FtsZ from Escherichia coli undergoes a strictly GTP-dependent polymerization and the polymers disappear as the GTP is consumed. Thus, FtsZ polymerization, like that of tubulin, is dynamic and regulated by GTP hydrolysis. These results provide the basis for the dynamics of the Z ring and favor a model in which the Z ring is formed by a nucleation event.
摘要
FtsZ形成一个细胞分裂环，称为Z环，它指导原核生物的细胞分裂。它与真核微管蛋白的序列相似性有限，并且与微管蛋白一样，它具有GTP酶活性以及组装成各种结构的能力，包括原丝、束和微环。通过使用电子显微镜和沉降法，我们证明来自大肠杆菌的FtsZ经历严格的GTP依赖性聚合，并且随着GTP的消耗聚合物消失。因此，FtsZ聚合，与微管蛋白一样，是动态的并且受GTP水解调节。这些结果为Z环的动态变化提供了基础，并支持Z环由成核事件形成的模型。
相似文献
1
Dynamic assembly of FtsZ regulated by GTP hydrolysis.由GTP水解调节的FtsZ动态组装。
EMBO J. 1998 Jan 15;17(2):462-9. doi: 10.1093/emboj/17.2.462.
2
Ca2+-mediated GTP-dependent dynamic assembly of bacterial cell division protein FtsZ into asters and polymer networks in vitro.钙离子介导的细菌细胞分裂蛋白FtsZ在体外进行GTP依赖的动态组装，形成星状体和聚合物网络。
EMBO J. 1997 Sep 1;16(17):5455-63. doi: 10.1093/emboj/16.17.5455.
3
Guanine nucleotide-dependent assembly of FtsZ into filaments.鸟嘌呤核苷酸依赖性FtsZ组装成丝状体。
J Bacteriol. 1994 May;176(9):2754-8. doi: 10.1128/jb.176.9.2754-2758.1994.
4
Assembly of an FtsZ mutant deficient in GTPase activity has implications for FtsZ assembly and the role of the Z ring in cell division.缺乏GTP酶活性的FtsZ突变体的组装对FtsZ组装以及Z环在细胞分裂中的作用具有重要意义。
J Bacteriol. 2001 Dec;183(24):7190-7. doi: 10.1128/JB.183.24.7190-7197.2001.
5
Assembly properties of the bacterial tubulin homolog FtsZ from the cyanobacterium sp. PCC 6803.来自集胞藻 PCC 6803 的细菌微管蛋白同源物 FtsZ 的组装特性。
J Biol Chem. 2019 Nov 1;294(44):16309-16319. doi: 10.1074/jbc.RA119.009621. Epub 2019 Sep 13.
6
Polymerization of Ftsz, a bacterial homolog of tubulin. is assembly cooperative?Ftsz（一种微管蛋白的细菌同源物）的聚合作用是否具有组装协同性？
J Biol Chem. 2001 Apr 13;276(15):11743-53. doi: 10.1074/jbc.M009033200. Epub 2001 Jan 4.
7
GTP-dependent polymerization of Escherichia coli FtsZ protein to form tubules.大肠杆菌FtsZ蛋白的GTP依赖性聚合形成微管。
Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5813-7. doi: 10.1073/pnas.91.13.5813.
8
Rate-limiting guanosine 5'-triphosphate hydrolysis during nucleotide turnover by FtsZ, a prokaryotic tubulin homologue involved in bacterial cell division.FtsZ是一种参与细菌细胞分裂的原核微管蛋白同源物，在核苷酸周转过程中，鸟苷5'-三磷酸水解起限速作用。
Biochemistry. 2004 Jan 13;43(1):282-8. doi: 10.1021/bi035465r.
9
FtsZ from Escherichia coli, Azotobacter vinelandii, and Thermotoga maritima--quantitation, GTP hydrolysis, and assembly.来自大肠杆菌、棕色固氮菌和嗜热栖热菌的FtsZ——定量、GTP水解及组装。
Cell Motil Cytoskeleton. 1998;40(1):71-86. doi: 10.1002/(SICI)1097-0169(1998)40:1<71::AID-CM7>3.0.CO;2-I.
10
Mutations in the bacterial cell division protein FtsZ highlight the role of GTP binding and longitudinal subunit interactions in assembly and function.细菌细胞分裂蛋白FtsZ中的突变突出了GTP结合和纵向亚基相互作用在组装和功能中的作用。
BMC Microbiol. 2015 Oct 13;15:209. doi: 10.1186/s12866-015-0544-z.
引用本文的文献
1
Distinct filament morphology and membrane tethering features of the dual FtsZ paralogs in Odinarchaeota.奥丁古菌中双FtsZ旁系同源物独特的丝状形态和膜系留特征。
EMBO J. 2025 Aug 8. doi: 10.1038/s44318-025-00529-7.
2
A FtsZ cis disassembly element acts in Z-ring assembly during bacterial cell division.一个FtsZ顺式解聚元件在细菌细胞分裂过程中的Z环组装中起作用。
Nat Commun. 2025 Jun 4;16(1):5194. doi: 10.1038/s41467-025-60517-7.
3
Unveiling Berberine analogues as potential inhibitors of Escherichia coli FtsZ through machine learning molecular docking and molecular dynamics approach.通过机器学习分子对接和分子动力学方法揭示小檗碱类似物作为大肠杆菌FtsZ的潜在抑制剂
Sci Rep. 2025 Apr 26;15(1):14668. doi: 10.1038/s41598-025-98835-x.
4
Differential GTP-dependent in-vitro polymerization of recombinant Physcomitrella FtsZ proteins.重组小立碗藓FtsZ蛋白的差异GTP依赖性体外聚合反应。
Sci Rep. 2025 Jan 24;15(1):3095. doi: 10.1038/s41598-024-85077-6.
5
Chemical genetic analysis of enoxolone inhibition of Clostridioides difficile toxin production reveals adenine deaminase and ATP synthase as antivirulence targets.甘草次酸抑制艰难梭菌毒素产生的化学遗传学分析揭示腺嘌呤脱氨酶和ATP合酶为抗毒力靶点。
J Biol Chem. 2024 Nov;300(11):107839. doi: 10.1016/j.jbc.2024.107839. Epub 2024 Sep 27.
6
N-terminus GTPase domain of the cytoskeleton protein FtsZ plays a critical role in its adaptation to high hydrostatic pressure.细胞骨架蛋白FtsZ的N端GTPase结构域在其适应高静水压过程中起关键作用。
Front Microbiol. 2024 Aug 16;15:1441398. doi: 10.3389/fmicb.2024.1441398. eCollection 2024.
7
Biomolecular condensate drives polymerization and bundling of the bacterial tubulin FtsZ to regulate cell division.生物分子凝聚物驱动细菌微管蛋白 FtsZ 的聚合和束状化，从而调节细胞分裂。
Nat Commun. 2023 Jun 28;14(1):3825. doi: 10.1038/s41467-023-39513-2.
8
Chlorogenic Acid Inhibits KM25 Growth and Proteolytic Activity in Fish-Based Products.绿原酸抑制鱼类制品中KM25的生长和蛋白水解活性。
Microorganisms. 2023 May 23;11(6):1367. doi: 10.3390/microorganisms11061367.
9
Gossypol acetate: A natural polyphenol derivative with antimicrobial activities against the essential cell division protein FtsZ.醋酸棉酚：一种天然多酚衍生物，对关键细胞分裂蛋白FtsZ具有抗菌活性。
Front Microbiol. 2023 Jan 12;13:1080308. doi: 10.3389/fmicb.2022.1080308. eCollection 2022.
10
Diversity and Potential Multifunctionality of Archaeal CetZ Tubulin-like Cytoskeletal Proteins.古菌 CetZ 微管蛋白样细胞骨架蛋白的多样性和潜在多功能性。
Biomolecules. 2023 Jan 9;13(1):134. doi: 10.3390/biom13010134.
本文引用的文献
1
Analysis of the interaction of FtsZ with itself, GTP, and FtsA.FtsZ与自身、GTP及FtsA之间相互作用的分析。
J Bacteriol. 1997 Sep;179(17):5551-9. doi: 10.1128/jb.179.17.5551-5559.1997.
2
FtsN, a late recruit to the septum in Escherichia coli.FtsN，一种在大肠杆菌中较晚定位于隔膜的蛋白。
Mol Microbiol. 1997 Jul;25(2):303-9. doi: 10.1046/j.1365-2958.1997.4641833.x.
3
Bacterial cell division and the Z ring.细菌细胞分裂与Z环
Annu Rev Biochem. 1997;66:93-116. doi: 10.1146/annurev.biochem.66.1.93.
4
Temperature shift experiments with an ftsZ84(Ts) strain reveal rapid dynamics of FtsZ localization and indicate that the Z ring is required throughout septation and cannot reoccupy division sites once constriction has initiated.使用ftsZ84（Ts）菌株进行的温度转换实验揭示了FtsZ定位的快速动态变化，并表明Z环在整个隔膜形成过程中都是必需的，并且一旦收缩开始就不能重新占据分裂位点。
J Bacteriol. 1997 Jul;179(13):4277-84. doi: 10.1128/jb.179.13.4277-4284.1997.
5
Inactivation of FtsI inhibits constriction of the FtsZ cytokinetic ring and delays the assembly of FtsZ rings at potential division sites.FtsI 的失活会抑制 FtsZ 细胞分裂环的收缩，并延迟 FtsZ 环在潜在分裂位点的组装。
Proc Natl Acad Sci U S A. 1997 Jan 21;94(2):559-64. doi: 10.1073/pnas.94.2.559.
6
Direct binding of FtsZ to ZipA, an essential component of the septal ring structure that mediates cell division in E. coli.FtsZ与ZipA直接结合，ZipA是大肠杆菌中介导细胞分裂的隔膜环结构的重要组成部分。
Cell. 1997 Jan 24;88(2):175-85. doi: 10.1016/s0092-8674(00)81838-3.
7
FtsA is localized to the septum in an FtsZ-dependent manner.FtsA以FtsZ依赖的方式定位于隔膜。
J Bacteriol. 1996 Dec;178(24):7167-72. doi: 10.1128/jb.178.24.7167-7172.1996.
8
FtsZ-spirals and -arcs determine the shape of the invaginating septa in some mutants of Escherichia coli.FtsZ螺旋和弧形结构决定了大肠杆菌某些突变体中内陷隔膜的形状。
Mol Microbiol. 1996 Oct;22(2):231-7. doi: 10.1046/j.1365-2958.1996.00100.x.
9
Colocalization of cell division proteins FtsZ and FtsA to cytoskeletal structures in living Escherichia coli cells by using green fluorescent protein.利用绿色荧光蛋白研究细胞分裂蛋白FtsZ和FtsA在活的大肠杆菌细胞中与细胞骨架结构的共定位。
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12998-3003. doi: 10.1073/pnas.93.23.12998.
10
FtsZ ring: the eubacterial division apparatus conserved in archaebacteria.FtsZ环：在古细菌中保守的真细菌分裂装置。
Mol Microbiol. 1996 Jul;21(2):313-9. doi: 10.1046/j.1365-2958.1996.6421360.x.
Zotero 插件