确定用于培养Thy-大肠杆菌菌株的最佳胸苷浓度。
Determining the optimal thymidine concentration for growing Thy- Escherichia coli strains.
作者信息
Molina F, Jiménez-Sánchez A, Guzmán E C
机构信息
Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, E-06080 Badajoz, Spain.
出版信息
J Bacteriol. 1998 Jun;180(11):2992-4. doi: 10.1128/JB.180.11.2992-2994.1998.
Abstract
Changes of thymidine concentration in the growth medium affect the chromosome replication time of Thy- strains without at the same time causing a detectable difference in the growth rate (R. H. Pritchard and A. Zaritsky, Nature 226:126-131, 1970). Consequently, the optimal thymidine concentration cannot be determined by ascertaining which concentration produces the highest growth rate. Here we present a method for determining the optimal thymidine concentration of any Thy- Escherichia coli strain. Using this method, we found that the E. coli "wild-type" strain MG1655 has a partial Thy- phenotype.
摘要
生长培养基中胸苷浓度的变化会影响胸苷营养缺陷型菌株的染色体复制时间，但同时不会导致生长速率出现可检测到的差异（R.H.普里查德和A.扎里茨基，《自然》226:126 - 131，1970）。因此，无法通过确定哪种浓度能产生最高生长速率来确定最佳胸苷浓度。在此，我们提出一种确定任何胸苷营养缺陷型大肠杆菌菌株最佳胸苷浓度的方法。使用该方法，我们发现大肠杆菌“野生型”菌株MG1655具有部分胸苷营养缺陷型表型。
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