Gindilis V, Goltsman E, Verlinsky Y
Reproductive Genetics Institute, Chicago, Illinois 60657, USA.
J Assist Reprod Genet. 1998 May;15(5):349-57. doi: 10.1023/a:1022517232580.
We performed multiple comparisons between available amino acid (aa) sequences of homeodomain(HOM)-containing proteins from a wide spectrum of animals to create an evolutionary classification of the proteins.
Based on results of statistical and special computational analyses of over 500 homeodomain aa sequences (HOMs) a novel system of concepts describing complex structural correlations between homologous proteins is proposed. This system includes such notions as differentiated isofunctionality of aa, chemotype, stereotype, local functional motifs, gradual conservativeness of aa positions, and group-specific domain patterns, as well as major categories of the evolutionary classification of HOMs (Division, Type, Branch, Class, Family, Series, Variety, Sort). Using this approach, a complete structural systematics of HOMs belonging to proteomes of eukaryotic animals is proposed.
The proposed structural classification of HOMs is in full agreement with the bulk of experimental data revealing complex functional similarities and differences among HOMs in terms of their expression patterns in developing embryos. It turn, this classification can provide answers regarding homology among homeodomains when experimental data are conflicting.
我们对来自广泛动物种类的含同源异型域（HOM）蛋白的可用氨基酸（aa）序列进行了多重比较，以创建这些蛋白的进化分类。
基于对500多个同源异型域氨基酸序列（HOMs）的统计和特殊计算分析结果，提出了一个描述同源蛋白之间复杂结构相关性的新的概念体系。该体系包括诸如氨基酸的分化同功能、化学型、刻板模式、局部功能基序、氨基酸位置的渐进保守性、组特异性结构域模式等概念，以及HOMs进化分类的主要类别（门、型、分支、类、家族、系列、变种、类别）。使用这种方法，提出了属于真核动物蛋白质组的HOMs的完整结构系统学。
所提出的HOMs结构分类与大量实验数据完全一致，这些数据揭示了HOMs在发育胚胎中的表达模式方面复杂的功能异同。反过来，当实验数据相互矛盾时，这种分类可以提供有关同源异型域之间同源性的答案。
