Klobusická M, Babusíková O
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Neoplasma. 1997;44(6):356-60.
This study reports the immunophenotypic features of a series of 62 selected acute leukemia patients with increased incidence of argyrophilic proteins (AgNORs) at the time of initial diagnosis. Peripheral blood and bone marrow cells of patients with T-ALL, B-precursor ALL and AML were studied. The method of silver staining was used to determine the number of AgNORs per cell. Cell surface markers were detected by a standard immunofluorescence assay. To demonstrate the relationship between AgNOR quantity and cell proliferation, the expression of activation and proliferation antigens CD38 and CD71 was investigated. To characterize the immunophenotype and the discrete stages of differentiation, the wide panel of antibodies against lymphoid, myeloid and non-lineage specific antigens was used. The number of AgNORs at diagnosis ranged from 3.05 to 6.70. Immunophenotypic analysis showed a variation in CD38 and CD71 expression among different leukemia subtypes. CD71 antigen was more expressed in T-ALL than in B-precursor ALL or in AML. Notable was the relationship between increased AgNOR quantity and antigens that characterize the immaturity of leukemic cells. The association with CD7, CD2, CD5 (without CD3 membrane expression) and CD34 in T blasts was evident. High positivity of CD19, CD10, CD34 and HLA-DR in relation to the increased amount of AgNORs in B-lineage ALL was observed. The vast majority of AML patients with high numbers of AgNORs simultaneously expressed CD13, CD33, CD34 and HLA-DR. One third of AML cases coexpressed T cell marker CD7. In conclusion, the presence of increased numbers of AgNORs at diagnosis might reflect the dependence on an early stage of leukemia cell differentiation.
本研究报告了一系列62例初诊时嗜银蛋白（AgNORs）发生率增加的急性白血病患者的免疫表型特征。对T淋巴细胞白血病（T-ALL）、前体B淋巴细胞白血病（B-precursor ALL）和急性髓系白血病（AML）患者的外周血和骨髓细胞进行了研究。采用银染法测定每个细胞的AgNORs数量。通过标准免疫荧光测定法检测细胞表面标志物。为了证明AgNOR数量与细胞增殖之间的关系，研究了活化和增殖抗原CD38和CD71的表达。为了表征免疫表型和分化的离散阶段，使用了针对淋巴细胞、髓细胞和非谱系特异性抗原的广泛抗体组。诊断时AgNORs的数量范围为3.05至6.70。免疫表型分析显示不同白血病亚型之间CD38和CD71表达存在差异。CD71抗原在T-ALL中的表达高于前体B淋巴细胞白血病或AML。值得注意的是AgNOR数量增加与表征白血病细胞不成熟的抗原之间的关系。T原始细胞中与CD7、CD2、CD5（无CD3膜表达）和CD34的关联很明显。观察到B系ALL中CD19、CD10、CD34和HLA-DR相对于AgNORs数量增加的高阳性率。绝大多数AgNORs数量高的AML患者同时表达CD13、CD33、CD34和HLA-DR。三分之一的AML病例共表达T细胞标志物CD7。总之，诊断时AgNORs数量增加可能反映了对白血病细胞分化早期阶段的依赖性。
