Klobusická M, Babusíková O
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Neoplasma. 1998;45(4):237-42.
In the present study we have examined immunophenotypic characteristics ofT-acute lymphoblastic leukemia (T-ALL) cells in relation to the expression of enzyme dipeptidyl peptidase IV (DPP IV). Peripheral blood and bone marrow cells of T-ALL patients at diagnosis were estimated. Cell surface markers were detected by a standard immunofluorescence assay and FACStar flow cytometry using a broad panel of monoclonal antibodies to define T-cell immunophenotype. DPP IV activity was investigated in phenotypically defined T-lymphoblasts. Association between DPP IV expression and proliferation was monitored by the expression of CD71 and CD38, which could be considered as markers of activation and proliferation, and by the silver-staining of nucleolar organizer regions-related proteins (argyrophilic proteins). Lymphoblasts, divided according to the presence or absence of DPP IV activity revealed remarkable heterogeneity in the immunophenotypic features. The vast majority of DPP IV positive T-ALL cases expressed CD4, CD8, CD7, CD5, CD2 along with CD71 and CD38 antigens, but the cells were surface membrane CD3 antigen negative. The phenotype of DPP IV negative cases displayed membrane CD3 antigen and variable expression of CD4 and CD8. CD71 and CD38 were frequently negative. It appears, that DPP IV active cells form the population with immature phenotype, as evidenced by mCD3 antigen absence. Relation between DPP IV positive cells and proliferation activity of T-blasts was observed, given by the presence of CD71 and CD38 positivity and overexpression of argyrophilic proteins (AgNORs). In conclusion, our study indicates a close relationship between DPP IV activity and the features ofT-cell immaturity. Association among DPP IV, CD71, CD38 and AgNORs might reflect possible relationship between immature phenotype and proliferative ability of blast cells in T-ALL patients.
在本研究中，我们检测了T急性淋巴细胞白血病（T-ALL）细胞的免疫表型特征，并将其与二肽基肽酶IV（DPP IV）的表达相关联。对T-ALL患者诊断时的外周血和骨髓细胞进行了评估。通过标准免疫荧光测定法和FACStar流式细胞术，使用一组广泛的单克隆抗体检测细胞表面标志物，以确定T细胞免疫表型。在表型确定的T淋巴母细胞中研究了DPP IV活性。通过CD71和CD38的表达监测DPP IV表达与增殖之间的关联，CD71和CD38可被视为激活和增殖的标志物，同时通过银染核仁组织区相关蛋白（嗜银蛋白）进行监测。根据DPP IV活性的有无划分的淋巴母细胞在免疫表型特征上显示出显著的异质性。绝大多数DPP IV阳性的T-ALL病例表达CD4、CD8、CD7、CD5、CD2以及CD71和CD38抗原，但细胞表面膜CD3抗原呈阴性。DPP IV阴性病例的表型显示膜CD3抗原以及CD4和CD8的可变表达。CD71和CD38通常为阴性。似乎DPP IV活性细胞形成了具有不成熟表型的群体，这通过mCD3抗原的缺失得以证明。观察到DPP IV阳性细胞与T淋巴母细胞增殖活性之间的关系，表现为CD71和CD38阳性以及嗜银蛋白（AgNORs）的过表达。总之，我们的研究表明DPP IV活性与T细胞不成熟特征之间存在密切关系。DPP IV、CD71、CD38和AgNORs之间的关联可能反映了T-ALL患者母细胞不成熟表型与增殖能力之间的可能关系。
