Zhang L, Skolnick J
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Protein Sci. 1998 May;7(5):1201-7. doi: 10.1002/pro.5560070515.
The Z-score of a protein is defined as the energy separation between the native fold and the average of an ensemble of misfolds in the units of the standard deviation of the ensemble. The Z-score is often used as a way of testing the knowledge-based potentials for their ability to recognize the native fold from other alternatives. However, it is not known what range of values the Z-scores should have if one had a correct potential. Here, we offer an estimate of Z-scores extracted from calorimetric measurements of proteins. The energies obtained from these experimental data are compared with those from computer simulations of a lattice model protein. It is suggested that the Z-scores calculated from different knowledge-based potentials are generally too small in comparison with the experimental values.
蛋白质的Z值定义为天然折叠与错误折叠集合体平均值之间的能量差，单位为该集合体的标准差。Z值常被用作一种测试基于知识的势函数从其他构象中识别天然折叠能力的方法。然而，如果拥有正确的势函数，Z值应处于何种范围尚不清楚。在此，我们提供了从蛋白质量热测量中提取的Z值估计。将从这些实验数据获得的能量与晶格模型蛋白质的计算机模拟能量进行了比较。结果表明，与实验值相比，从不同基于知识的势函数计算出的Z值通常过小。
