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虹鳟(Oncorhynchus mykiss)中两个鲑科重复DNA家族的特征

Characteristics of two salmonid repetitive DNA families in rainbow trout, Oncorhynchus mykiss.

作者信息

Reed K M, Dorschner M O, Phillips R B

机构信息

Department of Biological Sciences, University of Wisconsin-Milwaukee, 53201, USA.

出版信息

Cytogenet Cell Genet. 1997;79(3-4):184-7. doi: 10.1159/000134716.

Abstract

DNA sequence and genomic location of two repetitive DNA families in rainbow trout (Oncorhynchus mykiss) were investigated to develop molecular markers for chromosome identification. DNA fragments with sequences similar to the tandem and interspersed elements described in other salmonids were isolated. One clone showed differential hybridization to 12 pairs of chromosomes and should be a useful marker for physical mapping.

摘要

为开发用于染色体识别的分子标记,对虹鳟(Oncorhynchus mykiss)中两个重复DNA家族的DNA序列和基因组位置进行了研究。分离出了与其他鲑科鱼类中描述的串联和散布元件序列相似的DNA片段。一个克隆对12对染色体显示出差异杂交,应该是物理图谱构建的有用标记。

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