Sano H, Terashima Y
Faculty of Animal Science, Kitasato University, Towada, Japan.
Reprod Nutr Dev. 1998 Jan-Feb;38(1):81-91. doi: 10.1051/rnd:19980107.
Two glucose clamp techniques were performed for 6 h starting 2 h before the initiation of feeding to investigate the effect of dietary propionate supplementation on insulin responsiveness to glucose and tissue responsiveness to insulin in relation to feeding in rams. The rams were fed alfalfa hay without (Cont diet) and with 10 mmol.kg BW-1.d-1 of calcium propionate (Prop diet) for 4 weeks in randomized order. With the hyperglycemic clamp, the ratio of plasma insulin increment to glucose infusion rate did not differ between the diets, but for the Prop diet the ratio was less during the pre-feeding period. With the hyperinsulinemic euglycemic clamp, the glucose infusion rate was lower (P < 0.05) for the Prop diet than the Cont diet, and increased (P < 0.05) after feeding. In rams supplemented propionate tissue responsiveness to insulin was reduced. Propionate supplementation may either impair glucose utilization in response to insulin infusion or enhance glucose production from propionate.
在开始喂食前2小时起进行两种葡萄糖钳夹技术，持续6小时，以研究日粮中添加丙酸盐对成年公羊进食相关的胰岛素对葡萄糖反应性和组织对胰岛素反应性的影响。公羊随机顺序饲喂苜蓿干草4周，一组不添加丙酸盐（对照日粮），另一组添加10 mmol·kg体重-1·天-1的丙酸钙（丙酸盐日粮）。在高血糖钳夹试验中，日粮间血浆胰岛素增量与葡萄糖输注速率的比值无差异，但在丙酸盐日粮组，进食前该比值较低。在高胰岛素正常血糖钳夹试验中，丙酸盐日粮组的葡萄糖输注速率低于对照日粮组（P < 0.05），且进食后升高（P < 0.05）。在添加丙酸盐的成年公羊中，组织对胰岛素的反应性降低。添加丙酸盐可能会损害胰岛素输注后的葡萄糖利用，或增强丙酸盐的葡萄糖生成。
