Bobrovnik S A
A.V. Paladin Institute of Biochemistry, Ukraine, Kiev.
Ukr Biokhim Zh (1978). 1997 Sep-Dec;69(5-6):97-109.
It was shown that polyreactive properties of natural serum immunoglobulins or some monoclonal antibodies (i.e. their ability of binding to various antigenically dissimilar antigens) can be induced by the treatment with chaotropic ions (3.5 M KSCN, 5-30 min at 20 (C). Such transformation of monoclonal antibodies or serum immunoglobulins into polyreactive immunoglobulins (PRIG) seems to result of the changes in immunoglobulin conformation but is not from their unblocking. Interaction of PRIG with some antigens immobilized on the plate is strongly dependent on temperature unlike that of monospecific antibodies.
结果表明，天然血清免疫球蛋白或某些单克隆抗体的多反应性特性（即它们与各种抗原性不同的抗原结合的能力）可通过用离液序列高的离子（3.5 M KSCN，20℃下5 - 30分钟）处理来诱导。单克隆抗体或血清免疫球蛋白向多反应性免疫球蛋白（PRIG）的这种转变似乎是免疫球蛋白构象变化的结果，而非其去封闭的结果。与单特异性抗体不同，PRIG与固定在平板上的某些抗原的相互作用强烈依赖于温度。
