Kaliman P A, Zagaĭko A L, Shalamov R V, Ganusova G V, Barannik T V, Skripnik E V, Sokolik V V, Shabi B K
Kharkov State University.
Ukr Biokhim Zh (1978). 1997 Sep-Dec;69(5-6):138-48.
Cobalt chloride effect on rat liver and serum blood lipoproteins content and composition and on some characteristics of lipid peroxidation and oxidative stress was investigated. The activation of free-radical oxidation and oxidative stress development were judged from the dynamics of lipid peroxidation products accumulation, from cathepsin D unsedimental activity and from the alteration of microsomal cytochrome P-450 content and from activity of a number antioxidative enzymes. In order to evaluate the state of glutathione-defence system the activities of glutathione peroxidase, glutathione S-transferase, glutathione reductase and some NADPH-generating enzymes and reduced glutathione level alteration were studied in liver. The data obtained show that the cobalt chloride injection leads to the development of the oxidative stress and to activation of some antioxidant defence system, namely, glutathione-depending enzymes, and of microsomal cytochrome P-450 catabolism. The system blood lipoproteins (liver lipoproteins was found to participate in metabolism adaptation under oxidative stress and in maintenance of biological membranes structure and functioning.
研究了氯化钴对大鼠肝脏和血清血脂蛋白含量及组成，以及对脂质过氧化和氧化应激某些特征的影响。通过脂质过氧化产物积累的动态变化、组织蛋白酶D的非沉降活性、微粒体细胞色素P - 450含量的改变以及多种抗氧化酶的活性来判断自由基氧化的激活和氧化应激的发展。为了评估谷胱甘肽防御系统的状态，研究了肝脏中谷胱甘肽过氧化物酶、谷胱甘肽S -转移酶、谷胱甘肽还原酶和一些生成NADPH的酶的活性以及还原型谷胱甘肽水平的变化。所获得的数据表明，注射氯化钴会导致氧化应激的发展，并激活一些抗氧化防御系统，即依赖谷胱甘肽的酶和微粒体细胞色素P - 450分解代谢。发现系统血脂蛋白（肝脏脂蛋白）参与氧化应激下的代谢适应以及生物膜结构和功能的维持。
