Nieminen T, Käyhty H, Virolainen A, Eskola J
National Public Health Institute, Helsinki, Finland.
Vaccine. 1998 Jan-Feb;16(2-3):313-9. doi: 10.1016/s0264-410x(97)00162-x.
This study assessed the mucosal immune response in healthy adult volunteers immunized parenterally with either pneumococcal polysaccharide (N = 8) or pneumococcal polysaccharide-protein conjugate (N = 10) vaccine with an aim to evaluate the relevance of antibody secreting cell (ASC) response after parenteral vaccination. An ASC response to the four types of capsular polysaccharide tested was observed in all vaccinees 7-9 days after immunization. IgA was the predominant class in the ASC response, and IgG the next common, with very few IgM ASCs. The IgA/IgG ratio in the ASC response was higher after immunization with the polysaccharide than the conjugate vaccine. Antibodies of the IgA class were frequently seen in the saliva already before immunization; especially to serotypes 14 and 19F. A twofold increase of the type specific secretory IgA antibodies in saliva was found in eight of the 16 instances in which the specific IgA ASC response was > 100 ASC per 10(6) cells and in only one of the 52 instances with fewer ASCs. We conclude that the ASC response in the peripheral blood is a useful parameter of the antibody response to pneumococcal vaccines and a good indicator of a secretory IgA response in the saliva.
本研究评估了健康成年志愿者经肠道外接种肺炎球菌多糖疫苗（N = 8）或肺炎球菌多糖 - 蛋白结合疫苗（N = 10）后的黏膜免疫反应，旨在评估肠道外接种疫苗后抗体分泌细胞（ASC）反应的相关性。在免疫后7 - 9天，所有接种者均观察到对所检测的四种荚膜多糖的ASC反应。在ASC反应中，IgA是主要类别，其次是IgG，IgM ASC极少。与结合疫苗相比，多糖疫苗免疫后ASC反应中的IgA/IgG比值更高。在免疫前，唾液中就经常能检测到IgA类抗体；尤其是针对14型和19F型。在16例特异性IgA ASC反应>100个ASC/10⁶细胞的情况中，有8例唾液中特异性分泌型IgA抗体增加了两倍，而在52例ASC较少的情况中，只有1例出现这种情况。我们得出结论，外周血中的ASC反应是肺炎球菌疫苗抗体反应的一个有用参数，也是唾液中分泌型IgA反应的良好指标。
