Cai J, Winkler H H
Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688, USA.
Acta Virol. 1997 Oct;41(5):285-8.
The regulation of the citrate synthase (gltA) and ATP/ADP translocase (tlc) genes of the obligate intracellular bacterium, Rickettsia prowazekii, was analyzed in rickettsia-infected respiration-deficient G14 cells. The level of the gltA mRNAII and the tlc mRNA was much lower in the total RNA isolated from the infected G14 cells grown in 1 g/l glucose (low glucose, GL) medium than in that from infected G14 cells grown in 4.5 g/l glucose (high glucose, GH) medium. However, the level of the gltA mRNAI relative to 16 S rRNA was the same in GL and GH media. An increase in the level of the gltA mRNAII and the tlc mRNA could be observed as early as 2 hrs after shifting from GL to GH medium. We conclude that, under these experimental conditions, the tlc promoter and the gltA promoter P2, but not gltA promoter P1, were transcriptionally regulated.
对专性细胞内细菌普氏立克次体的柠檬酸合酶（gltA）基因和ATP/ADP转位酶（tlc）基因的调控，在感染立克次体的呼吸缺陷型G14细胞中进行了分析。从在1 g/l葡萄糖（低糖，GL）培养基中生长的感染G14细胞分离的总RNA中，gltA mRNAII和tlc mRNA的水平，比从在4.5 g/l葡萄糖（高糖，GH）培养基中生长的感染G14细胞分离的总RNA中的水平低得多。然而，在GL和GH培养基中，相对于16 S rRNA的gltA mRNAI水平是相同的。从GL培养基转换到GH培养基后，最早在2小时就可以观察到gltA mRNAII和tlc mRNA水平的增加。我们得出结论，在这些实验条件下，tlc启动子和gltA启动子P2，而非gltA启动子P1，受到转录调控。
