Cai J, Winkler H H
Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688, USA.
J Bacteriol. 1996 Sep;178(18):5543-5. doi: 10.1128/jb.178.18.5543-5545.1996.
Transcriptional regulation was demonstrated in Rickettsia prowazekii, an obligate intracytoplasmic bacterium. The level of citrate synthase (gltA) mRNA II, from promoter P2, was greater in the total RNA isolated from heavily infected L929 cells than in moderately infected L929 cells; conversely, the level of ATP/ADP translocase (tlc) mRNA was greater in moderately infected cells. The level of gltA mRNA I, from promoter P1, did not change under these conditions. The chemical half-lives of gltA mRNA II and tlc mRNA under these conditions were very similar.
转录调控在专性胞内细菌普氏立克次体中得到了证实。来自启动子P2的柠檬酸合酶(gltA)mRNA II在从重度感染的L929细胞中分离出的总RNA中的水平,高于中度感染的L929细胞;相反,ATP/ADP转位酶(tlc)mRNA的水平在中度感染的细胞中更高。来自启动子P1的gltA mRNA I的水平在这些条件下没有变化。在这些条件下,gltA mRNA II和tlc mRNA的化学半衰期非常相似。