Kataria R S, Tiwari A K, Gupta P K, Mehrotra M L, Rai A, Bandyopadhyay S K
National Biotechnology Centre, Bareilly, India.
Acta Virol. 1997 Dec;41(6):311-5.
Polymerase chain reaction (PCR) technique was applied to detect BHV-1 in bovine semen inoculated with BHV-1. The technique was found to be 10(6) times more sensitive than a non-isotopic dot-blot hybridization method in detecting viral genomic DNA. Of the three primer pairs used, the one chosen from glycoprotein gC appeared to be most sensitive as it could detect up to 0.01 TCID50 of BHV-1 in the semen. The technique could be useful in screening breeding bulls or samples of frozen semen prior to use in artificial insemination.
采用聚合酶链反应（PCR）技术检测接种了牛疱疹病毒1型（BHV-1）的牛精液中的BHV-1。结果发现，该技术在检测病毒基因组DNA方面比非同位素斑点杂交法敏感10^6倍。在所使用的三对引物中，从糖蛋白gC中选择的那对引物似乎最敏感，因为它能检测到精液中低至0.01半数组织培养感染剂量（TCID50）的BHV-1。该技术可用于在人工授精前筛选种公牛或冷冻精液样本。
