Sreenivasa B P, Rasool T J, Natarajan C
Indian Veterinary Research Institute, Bangalore, India.
Indian J Exp Biol. 1996 Nov;34(11):1169-71.
A pair of oligomers of 20 and 23 bp were designed for amplifying a 381 bp sequence from glycoprotein IV gene of bovine herpesvirus 1. The primer pairs were used for amplifying genomic DNA of BHV-1 directly from cell culture fluids under different experimental conditions such as, untreated cell culture fluid, thermal denaturation and proteinase K treatment in presence of detergent. The results reveal that direct thermal denaturation of cell culture fluid is sufficient to detect the virus by polymerase chain reaction.
设计了一对20个碱基对和23个碱基对的寡聚物，用于从牛疱疹病毒1糖蛋白IV基因中扩增出一段381个碱基对的序列。在不同实验条件下，如未经处理的细胞培养液、热变性以及在有去污剂存在的情况下用蛋白酶K处理，使用这些引物对直接从细胞培养液中扩增牛疱疹病毒1的基因组DNA。结果表明，细胞培养液直接热变性足以通过聚合酶链反应检测到该病毒。
