Rivera V M
ARIAD Gene Therapeutics, Cambridge, Massachusetts 02139, USA.
Methods. 1998 Apr;14(4):421-9. doi: 10.1006/meth.1998.0596.
Chemical inducers of dimerization (dimerizers) are a new class of reagents used for controlling protein-protein interactions. Use of a small-molecule, cell-permeable dimerizer to control the interaction of a transcription activation domain and a DNA-binding domain allows the level of expression of a target gene to be regulated by the concentration of dimerizer. The modular design of components has facilitated the development of multiple systems that can be used to readily generate cell lines in which basal expression of the target gene is low and induced to high levels by a nontoxic dimerizer. The development and use of one such system to control the expression of a gene in cultured cells are described.
化学二聚化诱导剂（二聚体形成剂）是用于控制蛋白质-蛋白质相互作用的一类新型试剂。使用小分子、细胞可渗透的二聚体形成剂来控制转录激活结构域和DNA结合结构域之间的相互作用，可使靶基因的表达水平受二聚体形成剂浓度的调控。各组分的模块化设计推动了多种系统的开发，这些系统可用于轻松构建细胞系，其中靶基因的基础表达水平较低，并可通过无毒的二聚体形成剂诱导至高水平。本文描述了利用这样一种系统在培养细胞中控制基因表达的开发与应用。
